Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12562 and 12563 are separated by 172 nucleotides12563 and 12564 are separated by 156 nucleotides12564 and 12565 are separated by 378 nucleotides12565 and 12566 are separated by 49 nucleotides mRNA_4194: 12562 - K01934 MTHFS 5-formyltetrahydrofolate cyclo-ligase, at 1,584,696 to 1,585,623 12562 mRNA_4195: 12563 - HMMPfam-Protein of unknown function (DUF2962)-PF11176, at 1,585,796 to 1,586,681 12563 mRNA_4196: 12564 - K12343 SRD5A1 3-oxo-5-alpha-steroid 4-dehydrogenase 1, at 1,586,838 to 1,587,953 12564 mRNA_4197: 12565 - K00512 CYP17A steroid 17alpha-monooxygenase / 17alpha-hydroxyprogesterone deacetylase, at 1,588,332 to 1,590,437 12565 mRNA_4198: 12566 - K00166 BCKDHA, bkdA1 2-oxoisovalerate dehydrogenase E1 component alpha subunit, at 1,590,487 to 1,592,266 12566
Group Condition mRNA_4194 mRNA_4195 mRNA_4196 mRNA_4197 mRNA_4198
12562 12563 12564 12565 12566
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. -0.4 -0.7 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.6 N.D. -0.5 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.2 -0.7 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.3 -0.4 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.4 -0.0 +0.0
growth on oleic acid YNB Glucose -0.2 N.D. +0.0 -0.3 -0.3
auxotrophy YNB Glucose -0.3 N.D. -0.3 -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.6 N.D. -0.3 +0.4 -0.1
auxotrophy Dropout Complete -0.1 N.D. -0.0 +0.1 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -0.2 -0.3 +0.2
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.6 -0.2 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -1.1 N.D. -0.7 +0.8 +0.7
growth on ricinoleic acid YNB Glucose -0.0 N.D. +0.1 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. -0.1 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.2 -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. -0.2 +0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.5 N.D. -0.2 +0.8 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. +0.1 +0.1 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. -0.2 -0.2 +0.5
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.2 +0.6 +0.4
growth on ypd YPD +0.5 N.D. +0.2 -0.1 +0.4
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