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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12562 and 12563 are separated by 172 nucleotides
12563 and 12564 are separated by 156 nucleotides
12564 and 12565 are separated by 378 nucleotides
12565 and 12566 are separated by 49 nucleotides
mRNA_4194: 12562 - K01934 MTHFS 5-formyltetrahydrofolate cyclo-ligase, at 1,584,696 to 1,585,623
12562
mRNA_4195: 12563 - HMMPfam-Protein of unknown function (DUF2962)-PF11176, at 1,585,796 to 1,586,681
12563
mRNA_4196: 12564 - K12343 SRD5A1 3-oxo-5-alpha-steroid 4-dehydrogenase 1, at 1,586,838 to 1,587,953
12564
mRNA_4197: 12565 - K00512 CYP17A steroid 17alpha-monooxygenase / 17alpha-hydroxyprogesterone deacetylase, at 1,588,332 to 1,590,437
12565
mRNA_4198: 12566 - K00166 BCKDHA, bkdA1 2-oxoisovalerate dehydrogenase E1 component alpha subunit, at 1,590,487 to 1,592,266
12566
Group
Condition
mRNA
_4194
mRNA
_4195
mRNA
_4196
mRNA
_4197
mRNA
_4198
12562
12563
12564
12565
12566
facs on lipid accumulation
Sorted High BODIPY Gate
-0.6
N.D.
-0.4
-0.7
-0.3
auxotrophy
YNB Glucose 75mM Methionine
-0.6
N.D.
-0.5
-0.2
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
N.D.
-0.2
-0.7
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
+0.2
N.D.
-0.3
-0.4
-0.3
auxotrophy
YNB Glucose 75mM Arginine
-0.3
N.D.
-0.4
-0.0
+0.0
growth on oleic acid
YNB Glucose
-0.2
N.D.
+0.0
-0.3
-0.3
auxotrophy
YNB Glucose
-0.3
N.D.
-0.3
-0.0
-0.2
ypd buoyancy
40 hrs Low Buoyancy
-0.6
N.D.
-0.3
+0.4
-0.1
auxotrophy
Dropout Complete
-0.1
N.D.
-0.0
+0.1
-0.5
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.2
N.D.
-0.2
-0.3
+0.2
growth on oleic acid
YNB Oleic Acid
-0.2
N.D.
+0.6
-0.2
-0.6
ypd buoyancy
40 hrs No Buoyancy Separation
-1.1
N.D.
-0.7
+0.8
+0.7
growth on ricinoleic acid
YNB Glucose
-0.0
N.D.
+0.1
-0.3
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.3
N.D.
-0.1
-0.3
-0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
N.D.
+0.2
-0.1
-0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.0
N.D.
-0.2
+0.1
+0.4
ypd buoyancy
40 hrs Median Buoyancy
-0.5
N.D.
-0.2
+0.8
+0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.2
N.D.
+0.1
+0.1
+0.5
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.5
N.D.
-0.2
-0.2
+0.5
ypd buoyancy
40 hrs High Buoyancy
-0.1
N.D.
-0.2
+0.6
+0.4
growth on ypd
YPD
+0.5
N.D.
+0.2
-0.1
+0.4
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