Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12570 and 12571 are separated by 2100 nucleotides12571 and 12572 are separated by 256 nucleotides12572 and 12573 are separated by 677 nucleotides12573 and 12574 are separated by 75 nucleotides mRNA_4202: 12570 - K00249 ACADM, acd acyl-CoA dehydrogenase, at 1,597,086 to 1,599,409 12570 mRNA_4203: 12571 - K19842 ROM1_2 RHO1 GDP-GTP exchange protein 1/2, at 1,601,510 to 1,605,241 12571 mRNA_4204: 12572 - K15292 STXBP1, MUNC18-1 syntaxin-binding protein 1, at 1,605,498 to 1,608,617 12572 mRNA_4205: 12573 - K09276 YBX1, NSEP1 Y-box-binding protein 1, at 1,609,295 to 1,611,072 12573 mRNA_4206: 12574 - Hypothetical Protein, at 1,611,148 to 1,611,771 12574
Group Condition mRNA_4202 mRNA_4203 mRNA_4204 mRNA_4205 mRNA_4206
12570 12571 12572 12573 12574
growth on ricinoleic acid YNB Ricinoleic Acid -0.9 -3.3 N.D. +0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 -3.2 N.D. +0.2 +0.1
growth on ricinoleic acid YNB Glucose -0.2 -2.7 N.D. +0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.9 N.D. -0.5 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.7 N.D. -0.3 -0.1
growth on oleic acid YNB Glucose -0.2 -0.8 N.D. +0.3 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.1 N.D. +0.0 -0.2
auxotrophy YNB Glucose +0.0 -1.4 N.D. +0.9 +0.1
growth on oleic acid YNB Oleic Acid -0.4 -0.2 N.D. +0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.5 N.D. +0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.4 -0.3 N.D. +0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.4 N.D. -0.0 +0.2
auxotrophy Dropout Complete -0.0 -0.7 N.D. +0.7 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.1 N.D. -0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.5 N.D. +0.5 +0.1
growth on ypd YPD +0.1 +0.1 N.D. -0.5 +0.4
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.7 N.D. +0.6 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.1 N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.4 N.D. -0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.4 +0.0 N.D. +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.6 N.D. -0.1 +0.4
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