Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12571 and 12572 are separated by 256 nucleotides12572 and 12573 are separated by 677 nucleotides12573 and 12574 are separated by 75 nucleotides12574 and 12575 overlap by 29 nucleotides mRNA_4203: 12571 - K19842 ROM1_2 RHO1 GDP-GTP exchange protein 1/2, at 1,601,510 to 1,605,241 12571 mRNA_4204: 12572 - K15292 STXBP1, MUNC18-1 syntaxin-binding protein 1, at 1,605,498 to 1,608,617 12572 mRNA_4205: 12573 - K09276 YBX1, NSEP1 Y-box-binding protein 1, at 1,609,295 to 1,611,072 12573 mRNA_4206: 12574 - Hypothetical Protein, at 1,611,148 to 1,611,771 12574 mRNA_4207: 12575 - BLAST similar to An07g08470 [Aspergillus kawachii IFO 4308], at 1,611,743 to 1,613,207 12575
Group Condition mRNA_4203 mRNA_4204 mRNA_4205 mRNA_4206 mRNA_4207
12571 12572 12573 12574 12575
growth on ricinoleic acid YNB Ricinoleic Acid -3.3 N.D. +0.1 +0.2 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -3.2 N.D. +0.2 +0.1 +0.4
growth on ricinoleic acid YNB Glucose -2.7 N.D. +0.4 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 N.D. -0.3 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.9 N.D. -0.5 +0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. +0.0 -0.2 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. +0.0 -0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.0 -0.1 -0.4
auxotrophy Dropout Complete -0.7 N.D. +0.7 +0.0 -0.6
growth on oleic acid YNB Glucose -0.8 N.D. +0.3 -0.0 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. -0.0 +0.2 -0.2
auxotrophy YNB Glucose -1.4 N.D. +0.9 +0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. +0.5 +0.1 -0.1
growth on ypd YPD +0.1 N.D. -0.5 +0.4 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. +0.2 +0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.2 +0.0 +0.0
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.3 +0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.7 N.D. +0.6 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. -0.1 +0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.3 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. -0.1 +0.4 -0.1
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