Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12582 and 12583 are separated by 704 nucleotides12583 and 12584 are separated by 148 nucleotides12584 and 12585 are separated by 318 nucleotides12585 and 12586 are separated by 517 nucleotides mRNA_4214: 12582 - Hypothetical Protein, at 1,627,604 to 1,628,234 12582 mRNA_4215: 12583 - HMMPfam-RhoGAP domain-PF00620,ProSiteProfiles-Rho GTPase-activating proteins domain profile.-PS50238,SMART-Fes/CIP4 homology domain-SM00055,SMART-GTPase-activator protein for Rho-like GTPases-SM00324,SUPERFAMILY--SSF103657,SUPERFAMILY--SSF48350, at 1,628,939 to 1,632,484 12583 mRNA_4216: 12584 - K13123 GPATCH1 G patch domain-containing protein 1, at 1,632,633 to 1,635,917 12584 mRNA_4217: 12585 - K14830 MAK11, PAK1IP1 protein MAK11, at 1,636,236 to 1,637,979 12585 mRNA_4218: 12586 - K17491 SMEK, PPP4R3 protein phosphatase 4 regulatory subunit 3, at 1,638,497 to 1,642,867 12586
Group Condition mRNA_4214 mRNA_4215 mRNA_4216 mRNA_4217 mRNA_4218
12582 12583 12584 12585 12586
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 +0.1 -0.5 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.2 -0.4 N.D. -0.2
auxotrophy Dropout Complete -0.4 -0.1 -0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.0 -0.0 N.D. -0.3
auxotrophy YNB Glucose -0.3 -0.0 -0.4 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.1 -0.3 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 -0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.0 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.1 +0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.0 +0.1 N.D. -0.1
growth on oleic acid YNB Oleic Acid +0.2 -0.1 -0.1 N.D. -0.2
growth on oleic acid YNB Glucose -0.0 +0.0 -0.1 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.1 -0.0 N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 -0.4 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 -0.1 N.D. -0.0
growth on ricinoleic acid YNB Glucose +0.1 -0.2 -0.1 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.0 -0.3 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.4 +0.2 N.D. -0.1
growth on ypd YPD +0.2 +0.0 +0.4 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.3 -0.5 N.D. +0.8
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.2 +0.4 N.D. +0.1
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