Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12584 and 12585 are separated by 318 nucleotides12585 and 12586 are separated by 517 nucleotides12586 and 12587 are separated by 124 nucleotides12587 and 12588 are separated by 231 nucleotides mRNA_4216: 12584 - K13123 GPATCH1 G patch domain-containing protein 1, at 1,632,633 to 1,635,917 12584 mRNA_4217: 12585 - K14830 MAK11, PAK1IP1 protein MAK11, at 1,636,236 to 1,637,979 12585 mRNA_4218: 12586 - K17491 SMEK, PPP4R3 protein phosphatase 4 regulatory subunit 3, at 1,638,497 to 1,642,867 12586 mRNA_4219: 12587 - HMMPfam-Protein phosphatase inhibitor 2 (IPP-2)-PF04979, at 1,642,992 to 1,644,310 12587 mRNA_4220: 12588 - K19932 NCS1 neuronal calcium sensor 1, at 1,644,542 to 1,645,935 12588
Group Condition mRNA_4216 mRNA_4217 mRNA_4218 mRNA_4219 mRNA_4220
12584 12585 12586 12587 12588
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. -0.4 +0.3 -3.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -0.1 -0.9 -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. -0.4 +0.3 -1.3
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. +0.2 -0.1 -1.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. -0.2 +0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.0 -0.3 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. -0.1 +0.2 -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. -0.1 -0.6 +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.3 -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. +0.2 +0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.1 N.D. +0.3 +0.2 -0.3
auxotrophy YNB Glucose -0.4 N.D. +0.2 +0.0 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.4 N.D. +0.1 +0.3 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. +0.8 +0.3 -0.5
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.2 +0.3 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.1 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.1 -0.5 +0.7
growth on oleic acid YNB Glucose -0.1 N.D. +0.1 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.1 -0.2 +0.6
auxotrophy Dropout Complete -0.3 N.D. +0.1 +0.2 +0.7
growth on ypd YPD +0.4 N.D. +0.1 +0.5 -0.2
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