Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12595 and 12596 are separated by 54 nucleotides12596 and 12597 are separated by 153 nucleotides12597 and 12598 are separated by 423 nucleotides12598 and 12599 are separated by 575 nucleotides mRNA_4227: 12595 - HMMPfam-F-box-like-PF12937,SUPERFAMILY--SSF48452, at 1,659,342 to 1,664,294 12595 mRNA_4228: 12596 - K00995 pgsA, PGS1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 1,664,349 to 1,666,570 12596 mRNA_4229: 12597 - K03145 TFIIS transcription elongation factor S-II, at 1,666,724 to 1,668,318 12597 mRNA_4230: 12598 - KOG1659 Class 2 transcription repressor NC2, alpha subunit (DRAP1), at 1,668,742 to 1,669,106 12598 mRNA_4231: 12599 - KOG4476 Gluconate transport-inducing protein, at 1,669,682 to 1,672,278 12599
Group Condition mRNA_4227 mRNA_4228 mRNA_4229 mRNA_4230 mRNA_4231
12595 12596 12597 12598 12599
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. N.D. -0.1
growth on oleic acid YNB Glucose -0.2 N.D. N.D. N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. N.D. N.D. +0.0
growth on ypd YPD +0.0 N.D. N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. N.D. +0.0
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. N.D. +0.0
growth on oleic acid YNB Oleic Acid +0.0 N.D. N.D. N.D. +0.1
auxotrophy YNB Glucose +0.0 N.D. N.D. N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. N.D. N.D. +0.1
auxotrophy Dropout Complete -0.0 N.D. N.D. N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. N.D. +0.2
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