Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12596 and 12597 are separated by 153 nucleotides12597 and 12598 are separated by 423 nucleotides12598 and 12599 are separated by 575 nucleotides12599 and 12600 are separated by 1660 nucleotides mRNA_4228: 12596 - K00995 pgsA, PGS1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 1,664,349 to 1,666,570 12596 mRNA_4229: 12597 - K03145 TFIIS transcription elongation factor S-II, at 1,666,724 to 1,668,318 12597 mRNA_4230: 12598 - KOG1659 Class 2 transcription repressor NC2, alpha subunit (DRAP1), at 1,668,742 to 1,669,106 12598 mRNA_4231: 12599 - KOG4476 Gluconate transport-inducing protein, at 1,669,682 to 1,672,278 12599 mRNA_4232: 12600 - K14832 MAK21, NOC1, CEBPZ ribosome biogenesis protein MAK21, at 1,673,939 to 1,677,855 12600
Group Condition mRNA_4228 mRNA_4229 mRNA_4230 mRNA_4231 mRNA_4232
12596 12597 12598 12599 12600
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. +0.0 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.0 N.D.
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.0 N.D.
growth on ypd YPD N.D. N.D. N.D. +0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +0.1 N.D.
auxotrophy YNB Glucose N.D. N.D. N.D. +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.2 N.D.
auxotrophy Dropout Complete N.D. N.D. N.D. +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.3 N.D.
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