Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12600 and 12601 are separated by 152 nucleotides12601 and 12602 are separated by 177 nucleotides12602 and 12603 are separated by 439 nucleotides12603 and 12604 are separated by 220 nucleotides mRNA_4232: 12600 - K14832 MAK21, NOC1, CEBPZ ribosome biogenesis protein MAK21, at 1,673,939 to 1,677,855 12600 mRNA_4233: 12601 - K11096 SNRPD2, SMD2 small nuclear ribonucleoprotein D2, at 1,678,008 to 1,678,995 12601 mRNA_4234: 12602 - SUPERFAMILY--SSF56112, at 1,679,173 to 1,681,802 12602 mRNA_4235: 12603 - ProSitePatterns-Tyrosine protein kinases specific active-site signature.-PS00109,SUPERFAMILY--SSF56112, at 1,682,242 to 1,685,142 12603 mRNA_4236: 12604 - HMMPfam-pinin/SDK/memA/ protein conserved region-PF04696, at 1,685,363 to 1,686,839 12604
Group Condition mRNA_4232 mRNA_4233 mRNA_4234 mRNA_4235 mRNA_4236
12600 12601 12602 12603 12604
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 -0.2 -2.0
auxotrophy Dropout Complete N.D. N.D. -0.2 -0.2 -0.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.1 +0.1 -1.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.5 -0.0 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 -0.1 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.4 +0.0 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.0 +0.5 -1.1
auxotrophy YNB Glucose N.D. N.D. -0.2 -0.1 -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.4 +0.1 -0.1
growth on ypd YPD N.D. N.D. -0.0 -0.0 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.2 +0.4 -0.7
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.2 -0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.0 -0.1 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.3 -0.0 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.0 +0.0 +0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.4 +0.0 +0.7
growth on oleic acid YNB Glucose N.D. N.D. -0.2 +0.1 +0.6
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.2 -0.2 +0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.3 +0.4 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 +0.1 +1.0
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