Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12601 and 12602 are separated by 177 nucleotides12602 and 12603 are separated by 439 nucleotides12603 and 12604 are separated by 220 nucleotides12604 and 12605 are separated by 674 nucleotides mRNA_4233: 12601 - K11096 SNRPD2, SMD2 small nuclear ribonucleoprotein D2, at 1,678,008 to 1,678,995 12601 mRNA_4234: 12602 - SUPERFAMILY--SSF56112, at 1,679,173 to 1,681,802 12602 mRNA_4235: 12603 - ProSitePatterns-Tyrosine protein kinases specific active-site signature.-PS00109,SUPERFAMILY--SSF56112, at 1,682,242 to 1,685,142 12603 mRNA_4236: 12604 - HMMPfam-pinin/SDK/memA/ protein conserved region-PF04696, at 1,685,363 to 1,686,839 12604 mRNA_4237: 12605 - K15100 SLC25A1, CTP solute carrier family 25 (mitochondrial citrate transporter), member 1, at 1,687,514 to 1,688,995 12605
Group Condition mRNA_4233 mRNA_4234 mRNA_4235 mRNA_4236 mRNA_4237
12601 12602 12603 12604 12605
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 +0.1 -1.1 -1.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 -0.2 -2.0 +0.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 +0.5 -1.1 -1.0
auxotrophy Dropout Complete N.D. -0.2 -0.2 -0.7 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.5 -0.0 -0.3 -0.0
growth on oleic acid YNB Glucose N.D. -0.2 +0.1 +0.6 -1.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 -0.2 +0.5 -1.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 +0.4 -0.7 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 +0.0 -0.3 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 -0.1 -0.1 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 -0.0 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 -0.1 +0.3 +0.3
growth on ricinoleic acid YNB Glucose N.D. -0.4 +0.1 -0.1 +1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 +0.0 +0.3 +0.3
auxotrophy YNB Glucose N.D. -0.2 -0.1 -0.2 +1.1
growth on oleic acid YNB Oleic Acid N.D. -0.4 +0.0 +0.7 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 +0.4 +0.4 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.2 +0.3 +1.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 -0.1 -0.6 +2.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.1 +1.0 +0.5
growth on ypd YPD N.D. -0.0 -0.0 -0.4 +2.2
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