Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12620 and 12621 are separated by 619 nucleotides12621 and 12622 are separated by 232 nucleotides12622 and 12623 are separated by 441 nucleotides12623 and 12624 are separated by 477 nucleotides mRNA_4252: 12620 - HMMPfam-Carboxymuconolactone decarboxylase family-PF02627,HMMPfam-Alpha/beta hydrolase family-PF12697,PRINTS-Alpha/beta hydrolase fold signature-PR00111,SUPERFAMILY--SSF53474,SUPERFAMILY--SSF69118, at 1,715,834 to 1,717,453 12620 mRNA_4253: 12621 - BLAST Dimeric alpha-beta barrel [Ceraceosorus bombacis], at 1,718,073 to 1,718,994 12621 mRNA_4254: 12622 - HMMPfam-Lactonase, 7-bladed beta-propeller-PF10282,SUPERFAMILY--SSF75011, at 1,719,227 to 1,720,870 12622 mRNA_4255: 12623 - HMMPfam-Dioxygenase-PF00775,SUPERFAMILY--SSF49482, at 1,721,312 to 1,723,112 12623 mRNA_4256: 12624 - HMMPfam-Putative serine esterase (DUF676)-PF05057,SUPERFAMILY--SSF53474, at 1,723,590 to 1,725,479 12624
Group Condition mRNA_4252 mRNA_4253 mRNA_4254 mRNA_4255 mRNA_4256
12620 12621 12622 12623 12624
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -1.3 -0.3 +0.2
growth on oleic acid YNB Glucose -0.2 N.D. -0.8 -0.0 +0.2
auxotrophy Dropout Complete +0.4 N.D. -1.1 +0.1 +0.0
auxotrophy YNB Glucose +0.1 N.D. -0.8 -0.0 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.2 -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. +0.3 +0.0 -0.1
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.5 -0.0 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. +0.4 -0.0 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.3 -0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +1.1 -0.4 -0.5
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. +0.1 -0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.4 +0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.3 +0.4 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. -0.5 +0.6 +0.4
growth on ypd YPD +0.1 N.D. +1.1 -0.4 -0.3
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.1 +0.5 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. +0.4 +0.5 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.7 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.6 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. +0.6 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. +0.3 +0.4 +0.3
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