Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12622 and 12623 are separated by 441 nucleotides12623 and 12624 are separated by 477 nucleotides12624 and 12625 are separated by 95 nucleotides12625 and 12626 are separated by 431 nucleotides mRNA_4254: 12622 - HMMPfam-Lactonase, 7-bladed beta-propeller-PF10282,SUPERFAMILY--SSF75011, at 1,719,227 to 1,720,870 12622 mRNA_4255: 12623 - HMMPfam-Dioxygenase-PF00775,SUPERFAMILY--SSF49482, at 1,721,312 to 1,723,112 12623 mRNA_4256: 12624 - HMMPfam-Putative serine esterase (DUF676)-PF05057,SUPERFAMILY--SSF53474, at 1,723,590 to 1,725,479 12624 mRNA_4257: 12625 - K00295 MTD1 methylenetetrahydrofolate dehydrogenase (NAD+), at 1,725,575 to 1,726,851 12625 mRNA_4258: 12626 - KOG4044 Mitochondrial associated endoribonuclease MAR1 (isochorismatase superfamily), at 1,727,283 to 1,728,545 12626
Group Condition mRNA_4254 mRNA_4255 mRNA_4256 mRNA_4257 mRNA_4258
12622 12623 12624 12625 12626
facs on lipid accumulation Sorted Low BODIPY Gate +1.1 -0.4 -0.5 -0.7 -1.1
auxotrophy Dropout Complete -1.1 +0.1 +0.0 -0.6 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.0 -0.1 +0.1 -1.4
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 -0.3 +0.2 -0.5 +1.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 +0.6 +0.4 -0.5 -0.8
growth on oleic acid YNB Oleic Acid -0.5 -0.0 +0.2 -0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.2 -0.3 -0.6 +0.2
auxotrophy YNB Glucose -0.8 -0.0 +0.2 -0.5 +0.4
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.3 +0.1 -0.8 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 +0.3 +0.1 -1.3 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.4 +0.3 -0.6 -0.7
growth on oleic acid YNB Glucose -0.8 -0.0 +0.2 +0.3 +0.2
growth on ricinoleic acid YNB Glucose -0.1 +0.5 +0.3 -0.3 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.1 +0.2 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.4 +0.3 -0.7 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.1 -0.1 +0.0 +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.0 +0.0 +0.1 -0.4
growth on ypd YPD +1.1 -0.4 -0.3 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 +0.2 +0.3 -0.0 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 +0.3 +0.2 -0.7 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.5 +0.4 -0.3 -0.3
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