Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12624 and 12625 are separated by 95 nucleotides12625 and 12626 are separated by 431 nucleotides12626 and 12627 are separated by 626 nucleotides12627 and 12628 are separated by 629 nucleotides mRNA_4256: 12624 - HMMPfam-Putative serine esterase (DUF676)-PF05057,SUPERFAMILY--SSF53474, at 1,723,590 to 1,725,479 12624 mRNA_4257: 12625 - K00295 MTD1 methylenetetrahydrofolate dehydrogenase (NAD+), at 1,725,575 to 1,726,851 12625 mRNA_4258: 12626 - KOG4044 Mitochondrial associated endoribonuclease MAR1 (isochorismatase superfamily), at 1,727,283 to 1,728,545 12626 mRNA_4259: 12627 - SUPERFAMILY--SSF51445, at 1,729,172 to 1,731,284 12627 mRNA_4260: 12628 - K11849 USP25_28, UBP2 ubiquitin carboxyl-terminal hydrolase 25/28, at 1,731,914 to 1,736,985 12628
Group Condition mRNA_4256 mRNA_4257 mRNA_4258 mRNA_4259 mRNA_4260
12624 12625 12626 12627 12628
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.7 -1.1 N.D. -0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.1 -1.4 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -1.3 -0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.6 -0.7 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.6 +0.2 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.5 -0.8 N.D. +0.3
growth on ypd YPD -0.3 -0.2 -0.0 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.1 -0.4 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.8 +0.2 N.D. +0.2
growth on oleic acid YNB Oleic Acid +0.2 -0.1 -0.4 N.D. +0.2
growth on ricinoleic acid YNB Glucose +0.3 -0.3 -0.5 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.7 +0.1 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -0.3 -0.3 N.D. +0.3
auxotrophy Dropout Complete +0.0 -0.6 +0.3 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.7 +0.3 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 +0.4 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.0 -0.5 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.1 +0.1 N.D. -0.1
auxotrophy YNB Glucose +0.2 -0.5 +0.4 N.D. +0.3
growth on oleic acid YNB Glucose +0.2 +0.3 +0.2 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.5 +1.1 N.D. +0.0
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