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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12624 and 12625 are separated by 95 nucleotides
12625 and 12626 are separated by 431 nucleotides
12626 and 12627 are separated by 626 nucleotides
12627 and 12628 are separated by 629 nucleotides
mRNA_4256: 12624 - HMMPfam-Putative serine esterase (DUF676)-PF05057,SUPERFAMILY--SSF53474, at 1,723,590 to 1,725,479
12624
mRNA_4257: 12625 - K00295 MTD1 methylenetetrahydrofolate dehydrogenase (NAD+), at 1,725,575 to 1,726,851
12625
mRNA_4258: 12626 - KOG4044 Mitochondrial associated endoribonuclease MAR1 (isochorismatase superfamily), at 1,727,283 to 1,728,545
12626
mRNA_4259: 12627 - SUPERFAMILY--SSF51445, at 1,729,172 to 1,731,284
12627
mRNA_4260: 12628 - K11849 USP25_28, UBP2 ubiquitin carboxyl-terminal hydrolase 25/28, at 1,731,914 to 1,736,985
12628
Group
Condition
mRNA
_4256
mRNA
_4257
mRNA
_4258
mRNA
_4259
mRNA
_4260
12624
12625
12626
12627
12628
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.5
-0.7
-1.1
N.D.
-0.6
facs on lipid accumulation
Sorted High BODIPY Gate
-0.1
+0.1
-1.4
N.D.
-0.4
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
-1.3
-0.2
N.D.
-0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.3
-0.6
-0.7
N.D.
+0.1
ypd buoyancy
40 hrs High Buoyancy
-0.3
-0.6
+0.2
N.D.
-0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.4
-0.5
-0.8
N.D.
+0.3
growth on ypd
YPD
-0.3
-0.2
-0.0
N.D.
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
+0.0
+0.1
-0.4
N.D.
-0.2
auxotrophy
YNB Glucose 75mM Arginine
+0.1
-0.8
+0.2
N.D.
+0.2
growth on oleic acid
YNB Oleic Acid
+0.2
-0.1
-0.4
N.D.
+0.2
growth on ricinoleic acid
YNB Glucose
+0.3
-0.3
-0.5
N.D.
+0.5
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.2
-0.7
+0.1
N.D.
+0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.4
-0.3
-0.3
N.D.
+0.3
auxotrophy
Dropout Complete
+0.0
-0.6
+0.3
N.D.
+0.3
auxotrophy
YNB Glucose 75mM Methionine
+0.3
-0.7
+0.3
N.D.
+0.2
ypd buoyancy
40 hrs Median Buoyancy
-0.1
+0.0
+0.4
N.D.
-0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.3
-0.0
-0.5
N.D.
+0.4
ypd buoyancy
40 hrs Low Buoyancy
+0.2
-0.1
+0.1
N.D.
-0.1
auxotrophy
YNB Glucose
+0.2
-0.5
+0.4
N.D.
+0.3
growth on oleic acid
YNB Glucose
+0.2
+0.3
+0.2
N.D.
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+0.2
-0.5
+1.1
N.D.
+0.0
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