Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12626 and 12627 are separated by 626 nucleotides12627 and 12628 are separated by 629 nucleotides12628 and 12629 are separated by 832 nucleotides12629 and 12630 are separated by 165 nucleotides mRNA_4258: 12626 - KOG4044 Mitochondrial associated endoribonuclease MAR1 (isochorismatase superfamily), at 1,727,283 to 1,728,545 12626 mRNA_4259: 12627 - SUPERFAMILY--SSF51445, at 1,729,172 to 1,731,284 12627 mRNA_4260: 12628 - K11849 USP25_28, UBP2 ubiquitin carboxyl-terminal hydrolase 25/28, at 1,731,914 to 1,736,985 12628 mRNA_4261: 12629 - HMMPfam-NUDIX domain-PF00293,ProSitePatterns-Nudix box signature.-PS00893,ProSiteProfiles-Nudix hydrolase domain profile.-PS51462,SUPERFAMILY--SSF55811, at 1,737,818 to 1,738,734 12629 mRNA_4262: 12630 - KOG2688 Transcription-associated recombination protein - Thp1p, at 1,738,900 to 1,741,067 12630
Group Condition mRNA_4258 mRNA_4259 mRNA_4260 mRNA_4261 mRNA_4262
12626 12627 12628 12629 12630
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 N.D. -0.6 -0.9 -1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 N.D. +0.1 -1.1 -1.4
facs on lipid accumulation Sorted High BODIPY Gate -1.4 N.D. -0.4 +0.3 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. -0.3 -1.3 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.1 -0.9 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.2 +0.1 -0.4
growth on oleic acid YNB Oleic Acid -0.4 N.D. +0.2 -0.2 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. +0.4 -0.3 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 N.D. +0.3 +0.6 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. +0.3 +0.6 -0.9
growth on ypd YPD -0.0 N.D. -0.1 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.3 +0.4 -0.8
ypd buoyancy 40 hrs No Buoyancy Separation +1.1 N.D. +0.0 -1.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. -0.1 +0.3 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.2 -0.2 +0.4
growth on oleic acid YNB Glucose +0.2 N.D. +0.1 +0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. -0.0 +0.7 +0.1
growth on ricinoleic acid YNB Glucose -0.5 N.D. +0.5 +0.7 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. +0.2 +0.4 +0.2
auxotrophy Dropout Complete +0.3 N.D. +0.3 +0.6 +0.4
auxotrophy YNB Glucose +0.4 N.D. +0.3 +0.9 +0.5
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