Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12639 and 12640 are separated by 305 nucleotides12640 and 12641 are separated by 386 nucleotides12641 and 12642 are separated by 244 nucleotides12642 and 12643 are separated by 242 nucleotides mRNA_4271: 12639 - K01196 AGL glycogen debranching enzyme, at 1,759,118 to 1,764,832 12639 mRNA_4272: 12640 - K02433 gatA, QRSL1 aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A, at 1,765,138 to 1,767,269 12640 mRNA_4273: 12641 - BLAST cellular morphogenesis-related protein [Monilioph..., at 1,767,656 to 1,771,232 12641 mRNA_4274: 12642 - K01720 prpD 2-methylcitrate dehydratase, at 1,771,477 to 1,773,946 12642 mRNA_4275: 12643 - HMMPfam-RhoGEF domain-PF00621,ProSiteProfiles-Dbl homology (DH) domain profile.-PS50010,SUPERFAMILY--SSF48065, at 1,774,189 to 1,777,372 12643
Group Condition mRNA_4271 mRNA_4272 mRNA_4273 mRNA_4274 mRNA_4275
12639 12640 12641 12642 12643
growth on ricinoleic acid YNB Ricinoleic Acid -2.2 N.D. -0.2 -0.3 +0.0
growth on oleic acid YNB Oleic Acid -0.5 N.D. -0.3 -1.8 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -0.3 -1.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -0.2 -0.7 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 N.D. -0.5 -0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 N.D. +0.3 -0.9 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -0.3 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. -0.5 -0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -0.1 +0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.2 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.1 +0.2 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. -0.7 +0.6 +0.4
growth on ypd YPD -0.0 N.D. +0.1 +0.0 -0.0
growth on oleic acid YNB Glucose -0.1 N.D. +0.3 +0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.3 +0.1 +0.1
auxotrophy Dropout Complete +0.1 N.D. +0.0 +0.4 +0.4
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.6 +0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.3 +0.3 +0.3
auxotrophy YNB Glucose +0.2 N.D. +0.2 +0.4 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 N.D. +0.1 +0.0 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.0 +0.7 +0.3
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