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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12639 and 12640 are separated by 305 nucleotides
12640 and 12641 are separated by 386 nucleotides
12641 and 12642 are separated by 244 nucleotides
12642 and 12643 are separated by 242 nucleotides
mRNA_4271: 12639 - K01196 AGL glycogen debranching enzyme, at 1,759,118 to 1,764,832
12639
mRNA_4272: 12640 - K02433 gatA, QRSL1 aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A, at 1,765,138 to 1,767,269
12640
mRNA_4273: 12641 - BLAST cellular morphogenesis-related protein [Monilioph..., at 1,767,656 to 1,771,232
12641
mRNA_4274: 12642 - K01720 prpD 2-methylcitrate dehydratase, at 1,771,477 to 1,773,946
12642
mRNA_4275: 12643 - HMMPfam-RhoGEF domain-PF00621,ProSiteProfiles-Dbl homology (DH) domain profile.-PS50010,SUPERFAMILY--SSF48065, at 1,774,189 to 1,777,372
12643
Group
Condition
mRNA
_4271
mRNA
_4272
mRNA
_4273
mRNA
_4274
mRNA
_4275
12639
12640
12641
12642
12643
growth on ricinoleic acid
YNB Ricinoleic Acid
-2.2
N.D.
-0.2
-0.3
+0.0
growth on oleic acid
YNB Oleic Acid
-0.5
N.D.
-0.3
-1.8
+0.3
facs on lipid accumulation
Sorted High BODIPY Gate
-0.2
N.D.
-0.3
-1.1
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+0.2
N.D.
-0.2
-0.7
-0.6
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.4
N.D.
-0.5
-0.1
-0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.5
N.D.
+0.3
-0.9
+0.2
facs on lipid accumulation
Sorted No BODIPY Gate
-0.3
N.D.
-0.3
-0.2
-0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.2
N.D.
-0.5
-0.2
-0.1
ypd buoyancy
40 hrs High Buoyancy
-0.2
N.D.
-0.1
+0.2
-0.1
ypd buoyancy
40 hrs Low Buoyancy
-0.1
N.D.
-0.2
+0.0
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.2
N.D.
-0.1
+0.2
-0.0
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.4
N.D.
-0.7
+0.6
+0.4
growth on ypd
YPD
-0.0
N.D.
+0.1
+0.0
-0.0
growth on oleic acid
YNB Glucose
-0.1
N.D.
+0.3
+0.2
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.2
N.D.
+0.3
+0.1
+0.1
auxotrophy
Dropout Complete
+0.1
N.D.
+0.0
+0.4
+0.4
growth on ricinoleic acid
YNB Glucose
+0.1
N.D.
+0.6
+0.2
+0.0
auxotrophy
YNB Glucose 75mM Methionine
+0.1
N.D.
+0.3
+0.3
+0.3
auxotrophy
YNB Glucose
+0.2
N.D.
+0.2
+0.4
+0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.7
N.D.
+0.1
+0.0
+0.3
auxotrophy
YNB Glucose 75mM Arginine
+0.2
N.D.
-0.0
+0.7
+0.3
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