Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12646 and 12647 are separated by 190 nucleotides12647 and 12648 are separated by 100 nucleotides12648 and 12649 are separated by 461 nucleotides12649 and 12650 are separated by 939 nucleotides mRNA_4278: 12646 - HMMPfam-Voltage-dependent anion channel-PF03595, at 1,786,017 to 1,788,218 12646 mRNA_4279: 12647 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 1,788,409 to 1,790,808 12647 mRNA_4280: 12648 - HMMPfam-Actin interacting protein 3-PF03915,SMART-Actin interacting protein 3-SM00806, at 1,790,909 to 1,794,329 12648 mRNA_4281: 12649 - K11778 DHDDS, RER2, SRT1 ditrans,polycis-polyprenyl diphosphate synthase, at 1,794,791 to 1,795,923 12649 mRNA_4282: 12650 - Hypothetical Protein, at 1,796,863 to 1,797,558 12650
Group Condition mRNA_4278 mRNA_4279 mRNA_4280 mRNA_4281 mRNA_4282
12646 12647 12648 12649 12650
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.1 -0.1 N.D. -1.8
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.3 +0.2 N.D. -0.6
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.3 -0.0 N.D. +0.1
growth on ypd YPD -0.1 -0.1 +0.0 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.1 -0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.2 +0.5 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.2 -0.3 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.3 +0.3 N.D. -0.3
auxotrophy YNB Glucose -0.2 -0.0 +0.6 N.D. -0.1
growth on oleic acid YNB Glucose +0.0 -0.1 +0.5 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.2 +0.7 N.D. -0.3
auxotrophy Dropout Complete -0.1 -0.0 +0.6 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.0 +0.0 N.D. +0.5
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.2 +0.5 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.1 +0.4 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.0 +0.5 N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.3 +0.2 +1.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.4 +0.3 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.4 +1.3 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.3 +1.3 N.D. -0.3
growth on oleic acid YNB Oleic Acid +0.1 -0.1 +1.1 N.D. +0.2
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