Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12647 and 12648 are separated by 100 nucleotides12648 and 12649 are separated by 461 nucleotides12649 and 12650 are separated by 939 nucleotides12650 and 12651 are separated by 306 nucleotides mRNA_4279: 12647 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 1,788,409 to 1,790,808 12647 mRNA_4280: 12648 - HMMPfam-Actin interacting protein 3-PF03915,SMART-Actin interacting protein 3-SM00806, at 1,790,909 to 1,794,329 12648 mRNA_4281: 12649 - K11778 DHDDS, RER2, SRT1 ditrans,polycis-polyprenyl diphosphate synthase, at 1,794,791 to 1,795,923 12649 mRNA_4282: 12650 - Hypothetical Protein, at 1,796,863 to 1,797,558 12650 mRNA_4283: 12651 - KOG2383 Predicted ATPase, at 1,797,865 to 1,800,888 12651
Group Condition mRNA_4279 mRNA_4280 mRNA_4281 mRNA_4282 mRNA_4283
12647 12648 12649 12650 12651
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.1 N.D. -1.8 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 N.D. -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.0 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.3 N.D. -0.3 -0.4
growth on ypd YPD -0.1 +0.0 N.D. -0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.2 N.D. -0.6 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.5 N.D. -0.1 -0.1
auxotrophy Dropout Complete -0.0 +0.6 N.D. -0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.0 N.D. +0.5 -0.1
growth on oleic acid YNB Glucose -0.1 +0.5 N.D. -0.1 -0.0
auxotrophy YNB Glucose -0.0 +0.6 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.4 N.D. +0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.3 N.D. +0.3 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.7 N.D. -0.3 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.5 N.D. +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.5 N.D. +0.4 +0.0
growth on ricinoleic acid YNB Glucose +0.2 +1.1 N.D. -0.3 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.3 N.D. +0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +1.3 N.D. -0.3 -0.1
growth on oleic acid YNB Oleic Acid -0.1 +1.1 N.D. +0.2 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +1.3 N.D. -0.2 +0.1
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