Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12652 and 12653 are separated by 63 nucleotides12653 and 12654 are separated by 92 nucleotides12654 and 12655 are separated by 184 nucleotides12655 and 12656 are separated by 129 nucleotides mRNA_4284: 12652 - KOG2806 Chitinase, at 1,801,203 to 1,803,513 12652 mRNA_4285: 12653 - K18162 NDUFAF5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5, at 1,803,577 to 1,805,345 12653 mRNA_4286: 12654 - K01288 KEX1 carboxypeptidase D, at 1,805,438 to 1,808,326 12654 mRNA_4287: 12655 - K14559 MPP10 U3 small nucleolar RNA-associated protein MPP10, at 1,808,511 to 1,811,471 12655 mRNA_4288: 12656 - Hypothetical Protein, at 1,811,601 to 1,812,350 12656
Group Condition mRNA_4284 mRNA_4285 mRNA_4286 mRNA_4287 mRNA_4288
12652 12653 12654 12655 12656
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -1.8 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -1.5 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. +0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. -0.3 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.7 N.D. +0.3
growth on ricinoleic acid YNB Glucose +0.0 N.D. +0.2 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. -0.3 N.D. +0.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. +0.7 N.D. -0.2
growth on ypd YPD +0.2 N.D. -0.1 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. -0.1 N.D. +0.8
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.3 N.D. +0.2
growth on oleic acid YNB Glucose +0.0 N.D. +0.0 N.D. +0.4
auxotrophy YNB Glucose +0.4 N.D. -0.4 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. -0.0 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. -0.3 N.D. +0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.6 N.D. +0.2 N.D. -0.1
auxotrophy Dropout Complete +0.3 N.D. +0.2 N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +0.7 N.D. +0.1
growth on oleic acid YNB Oleic Acid +0.3 N.D. +0.2 N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. -0.0 N.D. +0.6
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