Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12653 and 12654 are separated by 92 nucleotides12654 and 12655 are separated by 184 nucleotides12655 and 12656 are separated by 129 nucleotides12656 and 12657 are separated by 343 nucleotides mRNA_4285: 12653 - K18162 NDUFAF5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5, at 1,803,577 to 1,805,345 12653 mRNA_4286: 12654 - K01288 KEX1 carboxypeptidase D, at 1,805,438 to 1,808,326 12654 mRNA_4287: 12655 - K14559 MPP10 U3 small nucleolar RNA-associated protein MPP10, at 1,808,511 to 1,811,471 12655 mRNA_4288: 12656 - Hypothetical Protein, at 1,811,601 to 1,812,350 12656 mRNA_4289: 12657 - K15445 TRMT10A, TRM10, RG9MTD2 tRNA (guanine9-N1)-methyltransferase, at 1,812,694 to 1,814,089 12657
Group Condition mRNA_4285 mRNA_4286 mRNA_4287 mRNA_4288 mRNA_4289
12653 12654 12655 12656 12657
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.8 N.D. -0.3 -0.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.5 N.D. -0.4 -0.5
auxotrophy YNB Glucose 75mM Methionine N.D. -0.7 N.D. +0.3 -1.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 N.D. +0.6 -0.8
auxotrophy YNB Glucose N.D. -0.4 N.D. +0.5 -0.6
auxotrophy Dropout Complete N.D. +0.2 N.D. +0.4 -1.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -0.1 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 N.D. +0.2 -0.8
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 N.D. -0.1 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. +0.1 +0.3
growth on oleic acid YNB Glucose N.D. +0.0 N.D. +0.4 -0.3
growth on ypd YPD N.D. -0.1 N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 N.D. +0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 N.D. +0.8 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.2 N.D. +0.6 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 N.D. +0.8 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 N.D. +0.6 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. -0.0 +0.8
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.7 N.D. +0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.7 N.D. -0.2 +0.8
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