Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12660 and 12661 are separated by 1217 nucleotides12661 and 12662 are separated by 218 nucleotides12662 and 12663 are separated by 204 nucleotides12663 and 12664 are separated by 1503 nucleotides mRNA_4292: 12660 - HMMPfam-Glycosyl hydrolases family 16-PF00722,SUPERFAMILY--SSF49899, at 1,817,819 to 1,819,208 12660 mRNA_4293: 12661 - KOG1375 Beta tubulin, at 1,820,426 to 1,822,919 12661 mRNA_4294: 12662 - K08851 TP53RK, PRPK, BUD32 TP53 regulating kinase and related kinases, at 1,823,138 to 1,824,204 12662 mRNA_4295: 12663 - K15454 PUS9 tRNA pseudouridine synthase 9, at 1,824,409 to 1,826,962 12663 mRNA_4296: 12664 - Hypothetical Protein, at 1,828,466 to 1,828,997 12664
Group Condition mRNA_4292 mRNA_4293 mRNA_4294 mRNA_4295 mRNA_4296
12660 12661 12662 12663 12664
ypd buoyancy 40 hrs No Buoyancy Separation -2.0 N.D. N.D. +0.2 -2.0
facs on lipid accumulation Sorted Low BODIPY Gate -1.4 N.D. N.D. +0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.8 N.D. N.D. -0.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. N.D. -0.1 -0.5
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.2 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. N.D. -0.4 -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. N.D. -0.3 -0.7
auxotrophy YNB Glucose -0.3 N.D. N.D. -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.6 N.D. N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. N.D. -0.3 -0.2
growth on ypd YPD -0.5 N.D. N.D. +0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. N.D. +0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. N.D. -0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. N.D. -0.0 -0.3
growth on oleic acid YNB Oleic Acid -0.0 N.D. N.D. +0.2 -0.1
auxotrophy Dropout Complete +0.1 N.D. N.D. +0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. N.D. -0.1 +0.9
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. N.D. +0.1 +0.3
growth on ricinoleic acid YNB Glucose +0.5 N.D. N.D. +0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 N.D. N.D. +0.7 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +1.1 N.D. N.D. +0.5 -0.2
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