Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12661 and 12662 are separated by 218 nucleotides12662 and 12663 are separated by 204 nucleotides12663 and 12664 are separated by 1503 nucleotides12664 and 12665 are separated by 508 nucleotides mRNA_4293: 12661 - KOG1375 Beta tubulin, at 1,820,426 to 1,822,919 12661 mRNA_4294: 12662 - K08851 TP53RK, PRPK, BUD32 TP53 regulating kinase and related kinases, at 1,823,138 to 1,824,204 12662 mRNA_4295: 12663 - K15454 PUS9 tRNA pseudouridine synthase 9, at 1,824,409 to 1,826,962 12663 mRNA_4296: 12664 - Hypothetical Protein, at 1,828,466 to 1,828,997 12664 mRNA_4297: 12665 - K01166 E3.1.27.1 ribonuclease T2, at 1,829,506 to 1,831,349 12665
Group Condition mRNA_4293 mRNA_4294 mRNA_4295 mRNA_4296 mRNA_4297
12661 12662 12663 12664 12665
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 -2.0 +0.1
growth on oleic acid YNB Glucose N.D. N.D. -0.2 -0.6 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 -0.7 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.4 -0.7 +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 -0.4 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 -0.5 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.2 +0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.0 -0.3 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.3 -0.2 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 -0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.1 -0.3 +0.1
growth on ypd YPD N.D. N.D. +0.1 +0.0 -0.1
auxotrophy YNB Glucose N.D. N.D. -0.2 -0.1 +0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.2 -0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.5 -0.2 -0.0
auxotrophy Dropout Complete N.D. N.D. +0.0 -0.1 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 +0.3 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 -0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 +0.9 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 +0.1 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.7 -0.3 +0.2
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