Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12663 and 12664 are separated by 1503 nucleotides12664 and 12665 are separated by 508 nucleotides12665 and 12666 are separated by 137 nucleotides12666 and 12667 are separated by 228 nucleotides mRNA_4295: 12663 - K15454 PUS9 tRNA pseudouridine synthase 9, at 1,824,409 to 1,826,962 12663 mRNA_4296: 12664 - Hypothetical Protein, at 1,828,466 to 1,828,997 12664 mRNA_4297: 12665 - K01166 E3.1.27.1 ribonuclease T2, at 1,829,506 to 1,831,349 12665 mRNA_4298: 12666 - Hypothetical Protein, at 1,831,487 to 1,832,179 12666 mRNA_4299: 12667 - K12617 PATL1, PAT1 DNA topoisomerase 2-associated protein PAT1, at 1,832,408 to 1,835,999 12667
Group Condition mRNA_4295 mRNA_4296 mRNA_4297 mRNA_4298 mRNA_4299
12663 12664 12665 12666 12667
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.2 +0.0 N.D. -1.7
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -2.0 +0.1 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 -0.3 N.D. -1.2
growth on ypd YPD +0.1 +0.0 -0.1 N.D. -1.5
growth on oleic acid YNB Glucose -0.2 -0.6 -0.0 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.3 +0.0 N.D. -0.7
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.4 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.7 +0.4 N.D. +0.6
growth on oleic acid YNB Oleic Acid +0.2 -0.1 -0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.7 +0.2 N.D. +0.9
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.9 -0.3 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 -0.2 -0.0 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.3 +0.1 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.2 -0.1 +0.3 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.3 +0.1 N.D. +0.5
auxotrophy Dropout Complete +0.0 -0.1 +0.3 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.5 +0.2 N.D. +1.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 -0.2 +0.4 N.D. +0.9
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.1 +0.4 N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 -0.3 +0.2 N.D. +0.5
auxotrophy YNB Glucose -0.2 -0.1 +0.3 N.D. +1.6
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