Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12692 and 12693 are separated by 764 nucleotides12693 and 12694 are separated by 451 nucleotides12694 and 12695 are separated by 1230 nucleotides12695 and 12696 are separated by 266 nucleotides mRNA_4324: 12692 - K18754 LIN28 protein lin-28, at 1,909,910 to 1,911,534 12692 mRNA_4325: 12693 - K03231 EEF1A elongation factor 1-alpha, at 1,912,299 to 1,914,440 12693 mRNA_4326: 12694 - Hypothetical Protein, at 1,914,892 to 1,915,200 12694 mRNA_4327: 12695 - K18754 LIN28 protein lin-28, at 1,916,431 to 1,918,530 12695 mRNA_4328: 12696 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SUPERFAMILY--SSF81383, at 1,918,797 to 1,920,453 12696
Group Condition mRNA_4324 mRNA_4325 mRNA_4326 mRNA_4327 mRNA_4328
12692 12693 12694 12695 12696
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. +0.1 +0.0 -1.8
growth on oleic acid YNB Glucose -0.5 N.D. -0.4 +0.0 -0.4
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.4 -0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.1 -0.3 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 N.D. +0.0 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. -0.1 -0.1 -0.1
growth on ypd YPD -0.8 N.D. +0.2 +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.4 -0.2 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. +0.4 -0.3 -0.7
auxotrophy Dropout Complete -0.1 N.D. -0.2 -0.4 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. -0.5 -0.2 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.0 +0.0 -0.4
auxotrophy YNB Glucose +0.1 N.D. -0.2 -0.3 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. -0.5 -0.1 +0.6
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.1 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. -0.4 -0.3 +1.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. -0.2 -0.1 +0.8
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. +0.2 +0.2 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.1 -0.1 +0.7
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.2 -0.1 +1.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.2 -0.5 +1.9
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