Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12693 and 12694 are separated by 451 nucleotides12694 and 12695 are separated by 1230 nucleotides12695 and 12696 are separated by 266 nucleotides12696 and 12697 are separated by 677 nucleotides mRNA_4325: 12693 - K03231 EEF1A elongation factor 1-alpha, at 1,912,299 to 1,914,440 12693 mRNA_4326: 12694 - Hypothetical Protein, at 1,914,892 to 1,915,200 12694 mRNA_4327: 12695 - K18754 LIN28 protein lin-28, at 1,916,431 to 1,918,530 12695 mRNA_4328: 12696 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SUPERFAMILY--SSF81383, at 1,918,797 to 1,920,453 12696 mRNA_4329: 12697 - KOG1802 RNA helicase nonsense mRNA reducing factor (pNORF1), at 1,921,131 to 1,925,118 12697
Group Condition mRNA_4325 mRNA_4326 mRNA_4327 mRNA_4328 mRNA_4329
12693 12694 12695 12696 12697
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 +0.0 -1.8 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.4 -0.2 -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.3 -0.5 N.D.
growth on oleic acid YNB Glucose N.D. -0.4 +0.0 -0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 -0.2 -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.4 -0.3 -0.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 +0.0 -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.5 -0.2 +0.4 N.D.
auxotrophy YNB Glucose N.D. -0.2 -0.3 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 -0.2 -0.1 N.D.
auxotrophy Dropout Complete N.D. -0.2 -0.4 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 -0.1 +0.6 N.D.
growth on ypd YPD N.D. +0.2 +0.1 -0.2 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.2 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.1 +0.8 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.4 -0.3 +1.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.1 +0.7 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 -0.1 +1.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.2 +0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -0.5 +1.9 N.D.
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