Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12694 and 12695 are separated by 1230 nucleotides12695 and 12696 are separated by 266 nucleotides12696 and 12697 are separated by 677 nucleotides12697 and 12698 are separated by 590 nucleotides mRNA_4326: 12694 - Hypothetical Protein, at 1,914,892 to 1,915,200 12694 mRNA_4327: 12695 - K18754 LIN28 protein lin-28, at 1,916,431 to 1,918,530 12695 mRNA_4328: 12696 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SUPERFAMILY--SSF81383, at 1,918,797 to 1,920,453 12696 mRNA_4329: 12697 - KOG1802 RNA helicase nonsense mRNA reducing factor (pNORF1), at 1,921,131 to 1,925,118 12697 mRNA_4330: 12698 - K18738 HEK2, KHD1 heterogeneous nuclear rnp K-like protein 2, at 1,925,709 to 1,927,416 12698
Group Condition mRNA_4326 mRNA_4327 mRNA_4328 mRNA_4329 mRNA_4330
12694 12695 12696 12697 12698
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.0 -1.8 N.D. -0.9
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.3 -0.5 N.D. -1.4
auxotrophy Dropout Complete -0.2 -0.4 +0.3 N.D. -1.7
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.2 -0.0 N.D. -1.3
growth on oleic acid YNB Oleic Acid -0.4 -0.2 -0.3 N.D. -0.8
auxotrophy YNB Glucose -0.2 -0.3 +0.2 N.D. -1.3
growth on oleic acid YNB Glucose -0.4 +0.0 -0.4 N.D. -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.2 -0.1 N.D. -0.9
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -0.3 -0.7 N.D. -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 -0.1 N.D. -0.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.0 -0.4 N.D. -0.6
growth on ricinoleic acid YNB Glucose +0.1 -0.2 +0.2 N.D. -1.1
growth on ypd YPD +0.2 +0.1 -0.2 N.D. -0.7
ypd buoyancy 40 hrs Low Buoyancy -0.5 -0.2 +0.4 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 -0.1 +0.6 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.2 +0.4 N.D. -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 +0.8 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.3 +1.2 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 +0.7 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.1 +1.0 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.5 +1.9 N.D. +0.4
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