Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12695 and 12696 are separated by 266 nucleotides12696 and 12697 are separated by 677 nucleotides12697 and 12698 are separated by 590 nucleotides12698 and 12699 are separated by 221 nucleotides mRNA_4327: 12695 - K18754 LIN28 protein lin-28, at 1,916,431 to 1,918,530 12695 mRNA_4328: 12696 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SUPERFAMILY--SSF81383, at 1,918,797 to 1,920,453 12696 mRNA_4329: 12697 - KOG1802 RNA helicase nonsense mRNA reducing factor (pNORF1), at 1,921,131 to 1,925,118 12697 mRNA_4330: 12698 - K18738 HEK2, KHD1 heterogeneous nuclear rnp K-like protein 2, at 1,925,709 to 1,927,416 12698 mRNA_4331: 12699 - K15262 BCP1, BCCIP protein BCP1, at 1,927,638 to 1,928,975 12699
Group Condition mRNA_4327 mRNA_4328 mRNA_4329 mRNA_4330 mRNA_4331
12695 12696 12697 12698 12699
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -1.8 N.D. -0.9 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.5 N.D. -1.4 N.D.
auxotrophy Dropout Complete -0.4 +0.3 N.D. -1.7 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.0 N.D. -1.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.7 N.D. -0.6 N.D.
auxotrophy YNB Glucose -0.3 +0.2 N.D. -1.3 N.D.
growth on oleic acid YNB Oleic Acid -0.2 -0.3 N.D. -0.8 N.D.
growth on oleic acid YNB Glucose +0.0 -0.4 N.D. -0.8 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.1 N.D. -0.9 N.D.
growth on ricinoleic acid YNB Glucose -0.2 +0.2 N.D. -1.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. -0.8 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.4 N.D. -0.6 N.D.
growth on ypd YPD +0.1 -0.2 N.D. -0.7 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.4 N.D. -0.7 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.6 N.D. -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.4 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.8 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +1.2 N.D. -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.7 N.D. +0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 +1.0 N.D. +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +1.9 N.D. +0.4 N.D.
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