Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12697 and 12698 are separated by 590 nucleotides12698 and 12699 are separated by 221 nucleotides12699 and 12700 are separated by 387 nucleotides12700 and 12701 are separated by 64 nucleotides mRNA_4329: 12697 - KOG1802 RNA helicase nonsense mRNA reducing factor (pNORF1), at 1,921,131 to 1,925,118 12697 mRNA_4330: 12698 - K18738 HEK2, KHD1 heterogeneous nuclear rnp K-like protein 2, at 1,925,709 to 1,927,416 12698 mRNA_4331: 12699 - K15262 BCP1, BCCIP protein BCP1, at 1,927,638 to 1,928,975 12699 mRNA_4332: 12700 - K02912 RP-L32e, RPL32 large subunit ribosomal protein L32e, at 1,929,363 to 1,930,179 12700 mRNA_4333: 12701 - Hypothetical Protein, at 1,930,244 to 1,931,329 12701
Group Condition mRNA_4329 mRNA_4330 mRNA_4331 mRNA_4332 mRNA_4333
12697 12698 12699 12700 12701
auxotrophy Dropout Complete N.D. -1.7 N.D. N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.7 N.D. N.D. -0.6
growth on ricinoleic acid YNB Glucose N.D. -1.1 N.D. N.D. -0.2
auxotrophy YNB Glucose N.D. -1.3 N.D. N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -1.4 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.8 N.D. N.D. -0.5
auxotrophy YNB Glucose 75mM Methionine N.D. -1.3 N.D. N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.9 N.D. N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.9 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 N.D. N.D. -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.8 N.D. N.D. +0.2
growth on oleic acid YNB Glucose N.D. -0.8 N.D. N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.6 N.D. N.D. +0.2
growth on ypd YPD N.D. -0.7 N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 N.D. N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.6 N.D. N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 N.D. N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 N.D. N.D. +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 N.D. N.D. +0.2
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