Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12702 and 12703 are separated by 202 nucleotides12703 and 12704 are separated by 1338 nucleotides12704 and 12705 are separated by 1353 nucleotides12705 and 12706 are separated by 532 nucleotides mRNA_4334: 12702 - K10534 NR nitrate reductase (NAD(P)H), at 1,931,652 to 1,934,999 12702 mRNA_4335: 12703 - K12620 LSM1 U6 snRNA-associated Sm-like protein LSm1, at 1,935,202 to 1,935,968 12703 mRNA_4336: 12704 - K05863 SLC25A4S, ANT solute carrier family 25 (mitochondrial adenine nucleotide translocator), member 4/5/6/31, at 1,937,307 to 1,938,868 12704 mRNA_4337: 12705 - HMMPfam-Secretory lipase-PF03583,SUPERFAMILY--SSF53474, at 1,940,222 to 1,941,811 12705 mRNA_4338: 12706 - SUPERFAMILY--SSF53098, at 1,942,344 to 1,943,662 12706
Group Condition mRNA_4334 mRNA_4335 mRNA_4336 mRNA_4337 mRNA_4338
12702 12703 12704 12705 12706
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. -0.4 -0.7
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. N.D. -0.3 -0.1
auxotrophy YNB Glucose -0.2 N.D. N.D. -0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. -0.2 -0.2
auxotrophy Dropout Complete -0.0 N.D. N.D. -0.1 -0.1
growth on ypd YPD -0.1 N.D. N.D. -0.7 +0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. N.D. +0.5 -0.5
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. -0.1 -0.2
growth on oleic acid YNB Glucose -0.0 N.D. N.D. -0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.2 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. N.D. +0.4 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. N.D. +0.2 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. +0.0 +0.1
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. +0.2 -0.0
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. N.D. -0.3 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. N.D. -0.0 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. N.D. +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. +0.0 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. N.D. +0.2 +0.5
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