Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12703 and 12704 are separated by 1338 nucleotides12704 and 12705 are separated by 1353 nucleotides12705 and 12706 are separated by 532 nucleotides12706 and 12707 are separated by 177 nucleotides mRNA_4335: 12703 - K12620 LSM1 U6 snRNA-associated Sm-like protein LSm1, at 1,935,202 to 1,935,968 12703 mRNA_4336: 12704 - K05863 SLC25A4S, ANT solute carrier family 25 (mitochondrial adenine nucleotide translocator), member 4/5/6/31, at 1,937,307 to 1,938,868 12704 mRNA_4337: 12705 - HMMPfam-Secretory lipase-PF03583,SUPERFAMILY--SSF53474, at 1,940,222 to 1,941,811 12705 mRNA_4338: 12706 - SUPERFAMILY--SSF53098, at 1,942,344 to 1,943,662 12706 mRNA_4339: 12707 - K17677 IRC3 ATP-dependent helicase IRC3, at 1,943,840 to 1,947,247 12707
Group Condition mRNA_4335 mRNA_4336 mRNA_4337 mRNA_4338 mRNA_4339
12703 12704 12705 12706 12707
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.4 -0.7 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.3 -0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.2 -0.2 +0.0
auxotrophy YNB Glucose N.D. N.D. -0.0 -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.1 +0.2 -0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.1 -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.0 +0.1 -0.2
growth on ypd YPD N.D. N.D. -0.7 +0.7 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.0 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.4 -0.5 +0.1
auxotrophy Dropout Complete N.D. N.D. -0.1 -0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.3 +0.3 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.2 -0.4 +0.3
growth on oleic acid YNB Glucose N.D. N.D. -0.2 +0.3 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 +0.3 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.5 -0.5 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.0 +0.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 -0.3 +0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.2 -0.0 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.0 +0.3 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 +0.5 -0.2
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