Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12704 and 12705 are separated by 1353 nucleotides12705 and 12706 are separated by 532 nucleotides12706 and 12707 are separated by 177 nucleotides12707 and 12708 are separated by 423 nucleotides mRNA_4336: 12704 - K05863 SLC25A4S, ANT solute carrier family 25 (mitochondrial adenine nucleotide translocator), member 4/5/6/31, at 1,937,307 to 1,938,868 12704 mRNA_4337: 12705 - HMMPfam-Secretory lipase-PF03583,SUPERFAMILY--SSF53474, at 1,940,222 to 1,941,811 12705 mRNA_4338: 12706 - SUPERFAMILY--SSF53098, at 1,942,344 to 1,943,662 12706 mRNA_4339: 12707 - K17677 IRC3 ATP-dependent helicase IRC3, at 1,943,840 to 1,947,247 12707 mRNA_4340: 12708 - K01074 PPT palmitoyl-protein thioesterase, at 1,947,671 to 1,949,110 12708
Group Condition mRNA_4336 mRNA_4337 mRNA_4338 mRNA_4339 mRNA_4340
12704 12705 12706 12707 12708
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 -0.7 +0.1 -0.9
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 -0.2 +0.0 -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 -0.1 +0.0 -0.3
auxotrophy YNB Glucose N.D. -0.0 -0.1 -0.1 -0.3
auxotrophy Dropout Complete N.D. -0.1 -0.1 +0.1 -0.4
growth on ypd YPD N.D. -0.7 +0.7 -0.1 -0.3
growth on oleic acid YNB Glucose N.D. -0.2 +0.3 +0.1 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 +0.3 +0.0 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.1 -0.2 -0.2
growth on ricinoleic acid YNB Glucose N.D. -0.1 +0.3 -0.0 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.2 +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.0 -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 +0.3 +0.0 -0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.5 -0.2 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 +0.4 -0.1 -0.2
growth on oleic acid YNB Oleic Acid N.D. +0.2 -0.0 +0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 +0.2 -0.5 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.4 -0.5 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -0.4 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.3 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.5 -0.5 +0.2 +0.6
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