Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12710 and 12711 are separated by 498 nucleotides12711 and 12712 are separated by 520 nucleotides12712 and 12713 are separated by 590 nucleotides12713 and 12714 are separated by 763 nucleotides mRNA_4342: 12710 - K08801 PASK PAS domain containing serine/threonine kinase, at 1,951,654 to 1,955,244 12710 mRNA_4343: 12711 - K15206 TFC7 transcription factor C subunit 7, at 1,955,743 to 1,957,166 12711 mRNA_4344: 12712 - HMMPfam-Secretory lipase-PF03583,SUPERFAMILY--SSF53474, at 1,957,687 to 1,959,260 12712 mRNA_4345: 12713 - K04706 PIAS1 E3 SUMO-protein ligase PIAS1, at 1,959,851 to 1,962,070 12713 mRNA_4346: 12714 - K18754 LIN28 protein lin-28, at 1,962,834 to 1,965,398 12714
Group Condition mRNA_4342 mRNA_4343 mRNA_4344 mRNA_4345 mRNA_4346
12710 12711 12712 12713 12714
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.4 -0.6 -2.1 +0.1
auxotrophy Dropout Complete +0.1 +0.1 -0.2 -2.0 +0.1
auxotrophy YNB Glucose -0.1 -0.0 -0.3 -0.5 -0.0
growth on ypd YPD -0.0 +0.2 +0.4 -1.5 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 -0.1 -0.0 -1.3 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.1 -0.0 -0.9 +0.1
growth on ricinoleic acid YNB Glucose -0.0 -0.0 -0.4 +0.2 -0.4
growth on oleic acid YNB Oleic Acid +0.2 +0.1 -0.5 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.9 +0.3 -1.5 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.2 -0.2 -0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.0 -0.2 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.4 -0.2 -0.4 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.1 +0.2 -0.4 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.0 -0.1 +0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.1 -0.3 +0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.2 +0.3 -0.9 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.1 +0.2 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.8 +0.4 -1.1 -0.0
growth on oleic acid YNB Glucose +0.1 +0.4 -0.3 +0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.1 +0.2 +0.0 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.7 +0.6 -0.7 +0.0
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