Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12711 and 12712 are separated by 520 nucleotides12712 and 12713 are separated by 590 nucleotides12713 and 12714 are separated by 763 nucleotides12714 and 12715 are separated by 496 nucleotides mRNA_4343: 12711 - K15206 TFC7 transcription factor C subunit 7, at 1,955,743 to 1,957,166 12711 mRNA_4344: 12712 - HMMPfam-Secretory lipase-PF03583,SUPERFAMILY--SSF53474, at 1,957,687 to 1,959,260 12712 mRNA_4345: 12713 - K04706 PIAS1 E3 SUMO-protein ligase PIAS1, at 1,959,851 to 1,962,070 12713 mRNA_4346: 12714 - K18754 LIN28 protein lin-28, at 1,962,834 to 1,965,398 12714 mRNA_4347: 12715 - K03386 PRDX2_4, ahpC peroxiredoxin (alkyl hydroperoxide reductase subunit C), at 1,965,895 to 1,967,093 12715
Group Condition mRNA_4343 mRNA_4344 mRNA_4345 mRNA_4346 mRNA_4347
12711 12712 12713 12714 12715
auxotrophy Dropout Complete +0.1 -0.2 -2.0 +0.1 -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.6 -2.1 +0.1 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.0 -1.3 +0.0 -0.6
growth on oleic acid YNB Oleic Acid +0.1 -0.5 -0.4 +0.1 -1.1
auxotrophy YNB Glucose -0.0 -0.3 -0.5 -0.0 -1.0
growth on ricinoleic acid YNB Glucose -0.0 -0.4 +0.2 -0.4 -1.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.0 -0.9 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.9 +0.3 -1.5 -0.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.3 +0.0 -0.1 -0.7
growth on ypd YPD +0.2 +0.4 -1.5 +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.2 -0.4 +0.0 -0.8
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.2 -0.1 -0.1 -0.5
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.2 -0.4 +0.3 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.2 -0.2 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.3 -0.9 +0.4 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.1 +0.0 -0.1 -0.3
growth on oleic acid YNB Glucose +0.4 -0.3 +0.2 +0.2 -0.8
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 -0.1 -0.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.2 +0.0 +0.3 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 +0.6 -0.7 +0.0 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 +0.4 -1.1 -0.0 -0.0
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