Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12714 and 12715 are separated by 496 nucleotides12715 and 12716 are separated by 442 nucleotides12716 and 12717 are separated by 1108 nucleotides12717 and 12718 are separated by 322 nucleotides mRNA_4346: 12714 - K18754 LIN28 protein lin-28, at 1,962,834 to 1,965,398 12714 mRNA_4347: 12715 - K03386 PRDX2_4, ahpC peroxiredoxin (alkyl hydroperoxide reductase subunit C), at 1,965,895 to 1,967,093 12715 mRNA_4348: 12716 - KOG2265 Nuclear distribution protein NUDC, at 1,967,536 to 1,968,862 12716 mRNA_4349: 12717 - K02873 RP-L13e, RPL13 large subunit ribosomal protein L13e, at 1,969,971 to 1,971,273 12717 mRNA_4350: 12718 - K03635 MOCS2, moaE molybdopterin synthase catalytic subunit, at 1,971,596 to 1,972,460 12718
Group Condition mRNA_4346 mRNA_4347 mRNA_4348 mRNA_4349 mRNA_4350
12714 12715 12716 12717 12718
auxotrophy YNB Glucose -0.0 -1.0 N.D. -0.4 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.8 N.D. -0.5 -0.1
growth on ricinoleic acid YNB Glucose -0.4 -1.0 N.D. +0.2 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.5 N.D. -1.1 +0.3
auxotrophy Dropout Complete +0.1 -0.5 N.D. -0.7 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.7 N.D. -0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.3 N.D. -0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.7 N.D. +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.5 N.D. +0.3 +0.0
growth on oleic acid YNB Oleic Acid +0.1 -1.1 N.D. +0.9 -0.2
growth on oleic acid YNB Glucose +0.2 -0.8 N.D. +0.8 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.6 N.D. +0.6 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.5 N.D. +0.8 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 N.D. +0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.3 N.D. +0.6 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.3 N.D. +0.8 -0.1
growth on ypd YPD +0.1 -0.2 N.D. +0.5 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.6 N.D. +1.0 +0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.4 N.D. +0.9 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.4 N.D. +1.7 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.0 N.D. +2.1 +0.5
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