Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12729 and 12730 are separated by 1141 nucleotides12730 and 12731 are separated by 639 nucleotides12731 and 12732 are separated by 269 nucleotides12732 and 12733 are separated by 1250 nucleotides mRNA_4361: 12729 - K10576 UBE2H, UBC8 ubiquitin-conjugating enzyme E2 H, at 4,362 to 5,348 12729 mRNA_4362: 12730 - KOG0907 Thioredoxin, at 6,490 to 7,048 12730 mRNA_4363: 12731 - K19562 BIO3-BIO1 bifunctional dethiobiotin synthetase / adenosylmethionine---8-amino-7-oxononanoate aminotransferase, at 7,688 to 10,353 12731 mRNA_4364: 12732 - SUPERFAMILY--SSF48371, at 10,623 to 17,699 12732 mRNA_4365: 12733 - HMMPfam-Zinc finger, C3HC4 type (RING finger)-PF13920, at 18,950 to 21,856 12733
Group Condition mRNA_4361 mRNA_4362 mRNA_4363 mRNA_4364 mRNA_4365
12729 12730 12731 12732 12733
facs on lipid accumulation Sorted High BODIPY Gate -1.5 -0.5 N.D. -0.0 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.4 N.D. -1.5 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.8 -0.2 N.D. +0.1 -0.6
auxotrophy YNB Glucose +0.2 +0.3 N.D. -0.7 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 N.D. -0.5 -0.1
growth on ricinoleic acid YNB Glucose -0.2 +0.2 N.D. +0.0 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.2 N.D. -0.5 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.1 N.D. -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.2 N.D. -0.2 -0.1
growth on ypd YPD +0.9 -0.4 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.4 N.D. +0.3 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.3 N.D. +0.4 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 +0.3 N.D. +0.4 +0.2
auxotrophy Dropout Complete +0.1 +0.0 N.D. +0.6 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 +0.3 N.D. +1.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.3 N.D. -0.3 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.2 N.D. +1.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 +0.4 N.D. +0.9 -0.0
growth on oleic acid YNB Oleic Acid +0.2 +0.3 N.D. +0.2 +0.3
growth on oleic acid YNB Glucose -0.1 +0.5 N.D. +0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.3 N.D. +0.8 +0.4
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