Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12732 and 12733 are separated by 1250 nucleotides12733 and 12734 are separated by 354 nucleotides12734 and 12735 are separated by 78 nucleotides12735 and 12736 are separated by 97 nucleotides mRNA_4364: 12732 - SUPERFAMILY--SSF48371, at 10,623 to 17,699 12732 mRNA_4365: 12733 - HMMPfam-Zinc finger, C3HC4 type (RING finger)-PF13920, at 18,950 to 21,856 12733 mRNA_4366: 12734 - K11650 SMARCD SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D, at 22,211 to 24,480 12734 mRNA_4367: 12735 - K08856 STK16 serine/threonine kinase 16, at 24,559 to 26,572 12735 mRNA_4368: 12736 - K00652 bioF 8-amino-7-oxononanoate synthase, at 26,670 to 28,178 12736
Group Condition mRNA_4364 mRNA_4365 mRNA_4366 mRNA_4367 mRNA_4368
12732 12733 12734 12735 12736
facs on lipid accumulation Sorted Low BODIPY Gate -1.5 -0.5 N.D. -0.0 N.D.
auxotrophy YNB Glucose -0.7 -0.1 N.D. +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.5 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.1 N.D. -0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.5 -0.1 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Glucose +0.0 -0.3 N.D. -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.6 N.D. -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.5 +0.1 N.D. +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.6 N.D. -0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.1 N.D. -0.0 N.D.
growth on ypd YPD -0.2 -0.1 N.D. +0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.0 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.2 N.D. +0.1 N.D.
auxotrophy Dropout Complete +0.6 -0.2 N.D. +0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.2 +0.3 N.D. +0.3 N.D.
growth on oleic acid YNB Glucose +0.5 +0.1 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +1.0 -0.1 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.2 N.D. +0.8 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 -0.0 N.D. +0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.1 -0.2 N.D. +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 +0.4 N.D. +0.7 N.D.
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