Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12734 and 12735 are separated by 78 nucleotides12735 and 12736 are separated by 97 nucleotides12736 and 12737 are separated by 1074 nucleotides12737 and 12738 are separated by 405 nucleotides mRNA_4366: 12734 - K11650 SMARCD SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D, at 22,211 to 24,480 12734 mRNA_4367: 12735 - K08856 STK16 serine/threonine kinase 16, at 24,559 to 26,572 12735 mRNA_4368: 12736 - K00652 bioF 8-amino-7-oxononanoate synthase, at 26,670 to 28,178 12736 mRNA_4369: 12737 - K03671 trxA thioredoxin 1, at 29,253 to 29,815 12737 mRNA_4370: 12738 - HMMPfam-Purine nucleoside permease (NUP)-PF06516,PIRSF--PIRSF013171, at 30,221 to 32,027 12738
Group Condition mRNA_4366 mRNA_4367 mRNA_4368 mRNA_4369 mRNA_4370
12734 12735 12736 12737 12738
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 N.D. -2.9 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 N.D. -0.7 -0.4
growth on ricinoleic acid YNB Glucose N.D. -0.3 N.D. -0.3 -0.2
auxotrophy YNB Glucose N.D. +0.1 N.D. -0.5 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 N.D. -0.3 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 N.D. -0.8 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 N.D. +0.0 +0.0
growth on oleic acid YNB Glucose N.D. +0.2 N.D. -0.6 +0.1
auxotrophy Dropout Complete N.D. +0.3 N.D. +0.3 -0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 N.D. -0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.8 N.D. -1.5 +0.5
growth on oleic acid YNB Oleic Acid N.D. +0.3 N.D. -0.4 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. +0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 N.D. +0.5 -0.2
growth on ypd YPD N.D. +0.3 N.D. -0.5 +0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 N.D. +0.8 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 N.D. -0.6 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 N.D. +0.5 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 N.D. +0.8 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.6 N.D. +0.4 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.7 N.D. +0.2 +0.9
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