Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12738 and 12739 are separated by 484 nucleotides12739 and 12740 are separated by 3134 nucleotides12740 and 12741 are separated by 204 nucleotides12741 and 12742 are separated by 141 nucleotides mRNA_4370: 12738 - HMMPfam-Purine nucleoside permease (NUP)-PF06516,PIRSF--PIRSF013171, at 30,221 to 32,027 12738 mRNA_4371: 12739 - K10877 RAD54B DNA repair and recombination protein RAD54B, at 32,512 to 35,317 12739 mRNA_4372: 12740 - KOG2533 Permease of the major facilitator superfamily, at 38,452 to 41,065 12740 mRNA_4373: 12741 - SUPERFAMILY--SSF52058, at 41,270 to 42,990 12741 mRNA_4374: 12742 - K00232 E1.3.3.6, ACOX1, ACOX3 acyl-CoA oxidase, at 43,132 to 46,033 12742
Group Condition mRNA_4370 mRNA_4371 mRNA_4372 mRNA_4373 mRNA_4374
12738 12739 12740 12741 12742
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.6 -0.3 -0.3 -0.8
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.0 +0.3 +0.2 -2.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.1 +0.1 +0.5 -2.0
auxotrophy Dropout Complete -0.8 +0.4 -0.4 -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.3 -0.2 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 -0.3 +0.0 -0.2
growth on oleic acid YNB Oleic Acid -0.1 +0.1 +0.1 +0.3 -0.9
growth on oleic acid YNB Glucose +0.1 -0.2 -0.2 +0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.2 -0.1 -0.3 -0.2
growth on ypd YPD +0.6 -0.4 -0.4 -0.4 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.4 +0.1 -0.1 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 -0.1 +0.0 -0.2
growth on ricinoleic acid YNB Glucose -0.2 -0.1 +0.1 +0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 +0.1 -0.5 -0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.3 -0.2 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.5 +0.2 +0.0 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 +0.3 -0.3 -0.3 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 +0.2 -0.4 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.9 +0.0 -0.5 -0.1 -0.0
auxotrophy YNB Glucose -0.1 +0.3 +0.3 +0.0 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.2 +0.3 +0.2 -0.1
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