Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12754 and 12755 are separated by 355 nucleotides12755 and 12756 are separated by 459 nucleotides12756 and 12757 are separated by 358 nucleotides12757 and 12758 are separated by 711 nucleotides mRNA_4386: 12754 - BLAST polysaccharide lyase family 14 protein [Hypholoma sub..., at 70,020 to 71,335 12754 mRNA_4387: 12755 - HMMPfam-Ctf8-PF09696, at 71,691 to 72,315 12755 mRNA_4388: 12756 - KOG2084 Predicted histone tail methylase containing SET domain, at 72,775 to 74,031 12756 mRNA_4389: 12757 - K10735 GINS4, SLD5 GINS complex subunit 4, at 74,390 to 75,580 12757 mRNA_4390: 12758 - K16261 YAT yeast amino acid transporter, at 76,292 to 78,680 12758
Group Condition mRNA_4386 mRNA_4387 mRNA_4388 mRNA_4389 mRNA_4390
12754 12755 12756 12757 12758
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -2.5 -0.7 N.D. -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -1.5 -0.3 N.D. -0.4
auxotrophy Dropout Complete -0.5 -0.5 -0.9 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.6 -1.0 N.D. +0.6
growth on ricinoleic acid YNB Glucose -0.1 -0.9 -0.5 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.8 -0.4 N.D. -0.5
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.0 -0.9 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 -0.6 -0.9 N.D. +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 -0.2 -0.1 N.D. +0.2
auxotrophy YNB Glucose -0.4 -0.3 -0.4 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.9 -0.5 N.D. +0.8
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.2 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.1 -0.2 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.7 -0.2 N.D. +0.1
growth on ypd YPD -0.1 -0.5 +0.0 N.D. +0.8
growth on oleic acid YNB Glucose -0.3 +0.0 +0.1 N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.8 -0.1 N.D. +1.1
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.7 +0.1 N.D. -0.0
growth on oleic acid YNB Oleic Acid -0.1 -0.1 +0.4 N.D. +0.7
ypd buoyancy 40 hrs Low Buoyancy -0.0 +1.0 -0.4 N.D. +0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +1.5 +0.9 N.D. -0.4
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