Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12757 and 12758 are separated by 711 nucleotides12758 and 12759 are separated by 884 nucleotides12759 and 12760 overlap by 4 nucleotides12760 and 12761 are separated by 892 nucleotides mRNA_4389: 12757 - K10735 GINS4, SLD5 GINS complex subunit 4, at 74,390 to 75,580 12757 mRNA_4390: 12758 - K16261 YAT yeast amino acid transporter, at 76,292 to 78,680 12758 mRNA_4391: 12759 - Hypothetical Protein, at 79,565 to 80,188 12759 mRNA_4392: 12760 - KOG3069 Peroxisomal NUDIX hydrolase, at 80,185 to 81,389 12760 mRNA_4393: 12761 - K00029 E1.1.1.40, maeB malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+), at 82,282 to 84,844 12761
Group Condition mRNA_4389 mRNA_4390 mRNA_4391 mRNA_4392 mRNA_4393
12757 12758 12759 12760 12761
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 -0.7 -0.7 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.6 -0.5 +0.2 -0.7
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.0 -0.3 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 -0.8 +0.9 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 +0.4 -0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.6 +0.8 -0.4 +0.2
auxotrophy Dropout Complete N.D. +0.1 +0.2 -0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -0.5 +0.5 +0.3
auxotrophy YNB Glucose N.D. +0.3 +0.1 -0.0 -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.3 -0.3 +0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.7 +0.3 -0.0 -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.1 -0.1 +0.5
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 +0.4 +0.0 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.8 -0.6 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +0.0 +1.0 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.4 +0.2 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 -0.3 +1.2 -0.1
growth on oleic acid YNB Glucose N.D. +0.5 +0.1 +0.5 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.8 -0.1 +1.3 -0.3
growth on ypd YPD N.D. +0.8 +0.8 +0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +1.1 +0.2 +0.6 +0.3
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