Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12773 and 12774 are separated by 73 nucleotides12774 and 12775 are separated by 150 nucleotides12775 and 12776 are separated by 384 nucleotides12776 and 12777 are separated by 421 nucleotides mRNA_4405: 12773 - K05925 METTL3_14 mRNA (2'-O-methyladenosine-N6-)-methyltransferase, at 105,098 to 106,781 12773 mRNA_4406: 12774 - KOG2100 Dipeptidyl aminopeptidase, at 106,855 to 109,155 12774 mRNA_4407: 12775 - HMMPfam-Acetyltransferase (GNAT) family-PF00583,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 109,306 to 110,822 12775 mRNA_4408: 12776 - HMMPfam-Alcohol dehydrogenase GroES-like domain-PF08240,SUPERFAMILY--SSF50129,SUPERFAMILY--SSF51735, at 111,207 to 112,706 12776 mRNA_4409: 12777 - KOG1218 Proteins containing Ca2+-binding EGF-like domains, at 113,128 to 114,818 12777
Group Condition mRNA_4405 mRNA_4406 mRNA_4407 mRNA_4408 mRNA_4409
12773 12774 12775 12776 12777
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.0 -0.2 -0.5 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.0 -0.2 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.1 -0.3 -0.7 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.1 -0.3 -0.2 +0.0
growth on oleic acid YNB Oleic Acid +0.1 -0.2 +0.2 -0.6 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.2 -0.5 -0.1 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.0 -0.1 +0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.1 -0.0 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 -0.1 -0.2 +0.0 -0.2
growth on ricinoleic acid YNB Glucose +0.6 +0.1 -0.1 -0.5 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.4 -0.0 -0.6 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.3 +0.1 -0.6 -0.0
growth on oleic acid YNB Glucose +0.3 -0.1 +0.1 -0.4 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +1.0 -0.1 -0.3 +0.0 -0.3
growth on ypd YPD +0.2 +0.0 -0.1 +0.6 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.0 +0.4 -0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.4 -0.0 +0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +1.0 +0.1 -0.0 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.7 -0.1 +0.4 +0.2
auxotrophy YNB Glucose +0.6 +0.4 -0.1 +0.6 +0.1
auxotrophy Dropout Complete +0.5 +0.4 -0.0 +0.5 +0.3
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