Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12777 and 12778 are separated by 310 nucleotides12778 and 12779 are separated by 384 nucleotides12779 and 12780 are separated by 76 nucleotides12780 and 12781 are separated by 132 nucleotides mRNA_4409: 12777 - KOG1218 Proteins containing Ca2+-binding EGF-like domains, at 113,128 to 114,818 12777 mRNA_4410: 12778 - K00877 THI20 hydroxymethylpyrimidine/phosphomethylpyrimidine kinase / thiaminase, at 115,129 to 117,248 12778 mRNA_4411: 12779 - K14790 NOP9 nucleolar protein 9, at 117,633 to 120,365 12779 mRNA_4412: 12780 - SUPERFAMILY--SSF52047, at 120,442 to 126,387 12780 mRNA_4413: 12781 - KOG0252 Inorganic phosphate transporter, at 126,520 to 129,077 12781
Group Condition mRNA_4409 mRNA_4410 mRNA_4411 mRNA_4412 mRNA_4413
12777 12778 12779 12780 12781
growth on ypd YPD -0.2 +0.0 N.D. +0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.1 N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.0 N.D. -0.1 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.0 N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.2 N.D. +0.1 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.1 N.D. +0.1 +0.2
growth on ricinoleic acid YNB Glucose +0.0 +0.0 N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.2 N.D. +0.0 -0.1
auxotrophy YNB Glucose +0.1 -0.2 N.D. +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 N.D. +0.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.3 N.D. -0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.3 N.D. +0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 N.D. +0.1 +0.0
auxotrophy Dropout Complete +0.3 -0.1 N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.4 N.D. +0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.3 N.D. +0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 N.D. +0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.3 N.D. +0.1 -0.1
growth on oleic acid YNB Glucose +0.3 +0.2 N.D. +0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.3 N.D. +0.1 -0.0
growth on oleic acid YNB Oleic Acid +0.1 +0.4 N.D. +0.1 +0.3
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