Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12810 and 12811 are separated by 625 nucleotides12811 and 12812 are separated by 597 nucleotides12812 and 12813 are separated by 126 nucleotides12813 and 12814 are separated by 256 nucleotides mRNA_4442: 12810 - SUPERFAMILY--SSF52047, at 188,576 to 190,157 12810 mRNA_4443: 12811 - SUPERFAMILY--SSF52047, at 190,783 to 192,210 12811 mRNA_4444: 12812 - K14327 UPF2, RENT2 regulator of nonsense transcripts 2, at 192,808 to 197,198 12812 mRNA_4445: 12813 - K16342 PLA2G4, CPLA2 cytosolic phospholipase A2, at 197,325 to 199,939 12813 mRNA_4446: 12814 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 200,196 to 203,838 12814
Group Condition mRNA_4442 mRNA_4443 mRNA_4444 mRNA_4445 mRNA_4446
12810 12811 12812 12813 12814
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -1.1 N.D. -0.1 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 -0.3 N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.4 N.D. +0.0 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.3 N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.0 N.D. -0.1 -0.0
growth on ypd YPD -0.1 -0.3 N.D. +0.2 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.3 N.D. -0.1 -0.1
growth on ricinoleic acid YNB Glucose -0.0 +0.1 N.D. +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 N.D. -0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.1 N.D. +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.0 N.D. +0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.2 N.D. -0.2 +0.1
auxotrophy Dropout Complete +0.2 +0.2 N.D. +0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.0 N.D. +0.2 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.1 N.D. +0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.2 N.D. -0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.5 +0.4 N.D. -0.0 -0.3
growth on oleic acid YNB Glucose +0.1 +0.2 N.D. +0.2 +0.2
auxotrophy YNB Glucose +0.2 +0.1 N.D. +0.4 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.2 N.D. -0.0 +0.2
growth on oleic acid YNB Oleic Acid +0.1 +0.4 N.D. +0.3 +0.1
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