Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12812 and 12813 are separated by 126 nucleotides12813 and 12814 are separated by 256 nucleotides12814 and 12815 are separated by 220 nucleotides12815 and 12816 are separated by 89 nucleotides mRNA_4444: 12812 - K14327 UPF2, RENT2 regulator of nonsense transcripts 2, at 192,808 to 197,198 12812 mRNA_4445: 12813 - K16342 PLA2G4, CPLA2 cytosolic phospholipase A2, at 197,325 to 199,939 12813 mRNA_4446: 12814 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 200,196 to 203,838 12814 mRNA_4447: 12815 - K12666 OST1, RPN1 oligosaccharyltransferase complex subunit alpha (ribophorin I), at 204,059 to 206,130 12815 mRNA_4448: 12816 - KOG3979 FGF receptor activating protein 1, at 206,220 to 210,253 12816
Group Condition mRNA_4444 mRNA_4445 mRNA_4446 mRNA_4447 mRNA_4448
12812 12813 12814 12815 12816
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 -0.3 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 -0.4 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.1 N.D. -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 -0.1 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 +0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 -0.1 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 +0.2 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.0 N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 -0.0 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 +0.1 N.D. -0.1
auxotrophy Dropout Complete N.D. +0.2 -0.0 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 +0.1 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 +0.1 N.D. -0.1
auxotrophy YNB Glucose N.D. +0.4 +0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 +0.2 N.D. -0.0
growth on oleic acid YNB Oleic Acid N.D. +0.3 +0.1 N.D. +0.1
growth on oleic acid YNB Glucose N.D. +0.2 +0.2 N.D. +0.2
growth on ypd YPD N.D. +0.2 +0.2 N.D. +0.2
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