Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12814 and 12815 are separated by 220 nucleotides12815 and 12816 are separated by 89 nucleotides12816 and 12817 are separated by 90 nucleotides12817 and 12818 are separated by 186 nucleotides mRNA_4446: 12814 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 200,196 to 203,838 12814 mRNA_4447: 12815 - K12666 OST1, RPN1 oligosaccharyltransferase complex subunit alpha (ribophorin I), at 204,059 to 206,130 12815 mRNA_4448: 12816 - KOG3979 FGF receptor activating protein 1, at 206,220 to 210,253 12816 mRNA_4449: 12817 - K14168 CTU1, NCS6 cytoplasmic tRNA 2-thiolation protein 1, at 210,344 to 212,336 12817 mRNA_4450: 12818 - Hypothetical Protein, at 212,523 to 213,518 12818
Group Condition mRNA_4446 mRNA_4447 mRNA_4448 mRNA_4449 mRNA_4450
12814 12815 12816 12817 12818
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.2 -2.6 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. -0.3 -1.3 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.0 -0.8 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. -0.1 -0.4 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.6 -0.6 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.1 -0.3 -0.3
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.0 +0.0 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. +0.1 -0.1 -0.6
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.0 -0.5 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.1 -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.0 +0.5 -0.0
growth on ypd YPD +0.2 N.D. +0.2 +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. -0.0 +0.1 +0.2
auxotrophy YNB Glucose +0.1 N.D. -0.1 +0.4 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. -0.1 +0.5 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.1 +0.3 +0.4
auxotrophy Dropout Complete -0.0 N.D. +0.0 +0.4 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.0 +0.6 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -0.3 +1.3 +0.4
growth on oleic acid YNB Glucose +0.2 N.D. +0.2 +0.6 +0.7
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.1 +0.9 +0.6
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