Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12817 and 12818 are separated by 186 nucleotides12818 and 12819 are separated by 989 nucleotides12819 and 12820 are separated by 370 nucleotides12820 and 12821 are separated by 169 nucleotides mRNA_4449: 12817 - K14168 CTU1, NCS6 cytoplasmic tRNA 2-thiolation protein 1, at 210,344 to 212,336 12817 mRNA_4450: 12818 - Hypothetical Protein, at 212,523 to 213,518 12818 mRNA_4451: 12819 - K03355 APC8, CDC23 anaphase-promoting complex subunit 8, at 214,508 to 216,794 12819 mRNA_4452: 12820 - K13955 yogA zinc-binding alcohol dehydrogenase/oxidoreductase, at 217,165 to 218,716 12820 mRNA_4453: 12821 - KOG3855 Monooxygenase involved in coenzyme Q (ubiquinone) biosynthesis, at 218,886 to 222,303 12821
Group Condition mRNA_4449 mRNA_4450 mRNA_4451 mRNA_4452 mRNA_4453
12817 12818 12819 12820 12821
facs on lipid accumulation Sorted High BODIPY Gate -1.3 -0.6 N.D. -0.6 -0.1
ypd buoyancy 40 hrs Low Buoyancy -2.6 +0.0 N.D. +0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.2 N.D. -0.8 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.6 +0.0 N.D. -0.6 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 -0.4 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.6 N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.3 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Glucose +0.0 -0.6 N.D. +0.2 -0.0
growth on ypd YPD +0.1 -0.2 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 N.D. -0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.5 +0.4 N.D. -0.0 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.5 -0.0 N.D. -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.2 N.D. -0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.3 N.D. +0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.4 N.D. -0.0 +0.3
auxotrophy Dropout Complete +0.4 +0.5 N.D. +0.0 +0.2
auxotrophy YNB Glucose +0.4 +0.2 N.D. +0.4 +0.3
growth on oleic acid YNB Glucose +0.6 +0.7 N.D. +0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.6 +0.4 N.D. +0.4 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +1.3 +0.4 N.D. -0.1 +0.1
growth on oleic acid YNB Oleic Acid +0.9 +0.6 N.D. +0.3 +0.1
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