Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12854 and 12855 are separated by 719 nucleotides12855 and 12856 are separated by 336 nucleotides12856 and 12857 are separated by 215 nucleotides12857 and 12858 are separated by 436 nucleotides mRNA_4486: 12854 - K01293 E3.4.17.4 Gly-Xaa carboxypeptidase, at 296,577 to 298,255 12854 mRNA_4487: 12855 - K05857 PLCD phosphatidylinositol phospholipase C, delta, at 298,975 to 301,181 12855 mRNA_4488: 12856 - BLAST phospholipase C [Moesziomyces antarcticus T-34], at 301,518 to 302,496 12856 mRNA_4489: 12857 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 302,712 to 304,024 12857 mRNA_4490: 12858 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 304,461 to 305,865 12858
Group Condition mRNA_4486 mRNA_4487 mRNA_4488 mRNA_4489 mRNA_4490
12854 12855 12856 12857 12858
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.8 -0.4
growth on ypd YPD -0.1 N.D. N.D. -0.0 -0.3
auxotrophy YNB Glucose +0.3 N.D. N.D. -0.4 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. -0.1 +0.0
auxotrophy Dropout Complete +0.2 N.D. N.D. -0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. -0.4 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. N.D. +0.0 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. N.D. -0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. N.D. +0.5 +0.3
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. +0.1 -0.2
growth on oleic acid YNB Glucose +0.3 N.D. N.D. +0.0 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. N.D. -0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. +0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.5 N.D. N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. N.D. +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 N.D. N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. N.D. +0.2 +0.2
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