Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12855 and 12856 are separated by 336 nucleotides12856 and 12857 are separated by 215 nucleotides12857 and 12858 are separated by 436 nucleotides12858 and 12859 are separated by 225 nucleotides mRNA_4487: 12855 - K05857 PLCD phosphatidylinositol phospholipase C, delta, at 298,975 to 301,181 12855 mRNA_4488: 12856 - BLAST phospholipase C [Moesziomyces antarcticus T-34], at 301,518 to 302,496 12856 mRNA_4489: 12857 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 302,712 to 304,024 12857 mRNA_4490: 12858 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 304,461 to 305,865 12858 mRNA_4491: 12859 - Hypothetical Protein, at 306,091 to 307,232 12859
Group Condition mRNA_4487 mRNA_4488 mRNA_4489 mRNA_4490 mRNA_4491
12855 12856 12857 12858 12859
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.4 -0.2 -0.1
auxotrophy YNB Glucose N.D. N.D. -0.4 -0.3 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.8 -0.4 +0.6
auxotrophy Dropout Complete N.D. N.D. -0.1 -0.4 +0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.0 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.2 -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 -0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 -0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.1 -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 -0.2 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.0 -0.3 +0.4
growth on ypd YPD N.D. N.D. -0.0 -0.3 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 +0.0 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.2 +0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.2 +0.1 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 -0.2 +0.6
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.1 +0.0 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.3 -0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.5 +0.3 -0.1
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