Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12868 and 12869 are separated by 172 nucleotides12869 and 12870 are separated by 342 nucleotides12870 and 12871 are separated by 306 nucleotides12871 and 12872 are separated by 546 nucleotides mRNA_4500: 12868 - K14304 NUP85 nuclear pore complex protein Nup85, at 327,724 to 330,564 12868 mRNA_4501: 12869 - Hypothetical Protein, at 330,737 to 332,545 12869 mRNA_4502: 12870 - KOG3720 Lysosomal & prostatic acid phosphatases, at 332,888 to 334,792 12870 mRNA_4503: 12871 - K12397 AP3B AP-3 complex subunit beta, at 335,099 to 337,846 12871 mRNA_4504: 12872 - Hypothetical Protein, at 338,393 to 338,917 12872
Group Condition mRNA_4500 mRNA_4501 mRNA_4502 mRNA_4503 mRNA_4504
12868 12869 12870 12871 12872
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.2 +0.0 -2.0 -0.0
growth on ypd YPD +0.0 +0.0 +0.0 -0.7 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.1 +0.2 -0.3 -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.2 -0.1 -0.6 +0.2
growth on ricinoleic acid YNB Glucose +0.0 -0.2 -0.2 -0.3 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.2 -0.3 +0.0 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.1 +0.1 +0.3 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.1 +0.1 +0.0 +0.1
auxotrophy YNB Glucose +0.2 +0.3 +0.1 +0.5 -0.3
auxotrophy Dropout Complete +0.4 +0.3 +0.1 -0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.4 +0.0 -0.3 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.6 +0.0 +0.5 -0.5
growth on oleic acid YNB Glucose +0.4 +0.2 +0.3 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.5 +0.1 +0.4 -0.1
growth on oleic acid YNB Oleic Acid +0.4 +0.4 +0.1 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.3 -0.1 -0.1 +0.6
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.0 -0.1 +0.5 +0.9
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.0 -0.2 +0.5 +1.0
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.3 +0.1 +0.7 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.2 +0.3 +0.8 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.3 +0.3 -0.1 +1.3
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