Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12904 and 12905 are separated by 375 nucleotides12905 and 12906 are separated by 139 nucleotides12906 and 12907 are separated by 80 nucleotides12907 and 12908 are separated by 181 nucleotides mRNA_4536: 12904 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 404,129 to 405,942 12904 mRNA_4537: 12905 - KOG0472 Leucine-rich repeat protein, at 406,318 to 409,422 12905 mRNA_4538: 12906 - KOG2112 Lysophospholipase, at 409,562 to 410,663 12906 mRNA_4539: 12907 - HMMPfam-DNA polymerase subunit Cdc27-PF09507, at 410,744 to 412,303 12907 mRNA_4540: 12908 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 412,485 to 416,287 12908
Group Condition mRNA_4536 mRNA_4537 mRNA_4538 mRNA_4539 mRNA_4540
12904 12905 12906 12907 12908
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.1 -1.8 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 +0.4 -1.5 N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.2 +0.1 N.D. -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.2 -0.0 N.D. -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.0 +0.8 N.D. -0.6
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.2 +0.1 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy +0.5 -0.1 -0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.7 +0.6 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 +0.1 N.D. -0.2
auxotrophy Dropout Complete +0.1 -0.2 +0.2 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.2 +0.2 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.1 +0.1 N.D. -0.0
growth on ypd YPD -0.4 +0.3 +0.7 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.0 -0.6 N.D. +0.4
growth on oleic acid YNB Glucose +0.1 +0.0 +0.4 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 +0.4 +0.1 N.D. +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.3 +0.8 N.D. -0.0
growth on oleic acid YNB Oleic Acid +0.0 +0.0 +0.5 N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.0 +0.4 +0.0 N.D. +0.3
auxotrophy YNB Glucose -0.0 +0.2 +0.7 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.3 +1.3 N.D. -0.8
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