Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12912 and 12913 are separated by 708 nucleotides12913 and 12914 are separated by 286 nucleotides12914 and 12915 are separated by 1213 nucleotides12915 and 12916 are separated by 380 nucleotides mRNA_4544: 12912 - K01251 E3.3.1.1, ahcY adenosylhomocysteinase, at 424,792 to 427,173 12912 mRNA_4545: 12913 - K11088 SNRPD3, SMD3 small nuclear ribonucleoprotein D3, at 427,882 to 428,716 12913 mRNA_4546: 12914 - K16576 ACTR10, ARP11 actin-related protein 10, at 429,003 to 431,242 12914 mRNA_4547: 12915 - ProSiteProfiles-SET domain profile.-PS50280,SUPERFAMILY--SSF82199, at 432,456 to 433,199 12915 mRNA_4548: 12916 - K03392 ACMSD aminocarboxymuconate-semialdehyde decarboxylase, at 433,580 to 435,269 12916
Group Condition mRNA_4544 mRNA_4545 mRNA_4546 mRNA_4547 mRNA_4548
12912 12913 12914 12915 12916
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -1.5 -0.9
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -2.1 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -1.4 -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 -0.8
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.1 -0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.3 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.4 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.3 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.0 +0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.6 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.1 +0.3
auxotrophy Dropout Complete N.D. N.D. N.D. +0.0 +0.4
growth on ypd YPD N.D. N.D. N.D. +0.7 -0.2
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.6 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.4 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.7 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +1.1 -0.1
auxotrophy YNB Glucose N.D. N.D. N.D. +1.0 +0.5
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