Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12919 and 12920 are separated by 425 nucleotides12920 and 12921 are separated by 948 nucleotides12921 and 12922 are separated by 122 nucleotides12922 and 12923 are separated by 639 nucleotides mRNA_4551: 12919 - HMMPfam-Whi5 like-PF08528, at 440,384 to 442,291 12919 mRNA_4552: 12920 - KOG2263 Methionine synthase II (cobalamin-independent), at 442,717 to 444,581 12920 mRNA_4553: 12921 - SUPERFAMILY--SSF81383, at 445,530 to 447,635 12921 mRNA_4554: 12922 - K12585 DIS3, RRP44 exosome complex exonuclease DIS3/RRP44, at 447,758 to 451,562 12922 mRNA_4555: 12923 - KOG3855 Monooxygenase involved in coenzyme Q (ubiquinone) biosynthesis, at 452,202 to 455,003 12923
Group Condition mRNA_4551 mRNA_4552 mRNA_4553 mRNA_4554 mRNA_4555
12919 12920 12921 12922 12923
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.5 -0.3 N.D. -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.1 -0.5 N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.4 -0.1 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.3 -0.5 +0.1 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.4 +0.0 N.D. +0.2
auxotrophy Dropout Complete +0.5 -0.3 -0.3 N.D. -0.1
auxotrophy YNB Glucose +0.6 -0.6 +0.1 N.D. -0.1
growth on oleic acid YNB Glucose +0.1 +0.2 +0.0 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.0 -0.0 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 +0.2 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.2 -0.1 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.0 +0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.1 -0.1 N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.2 -0.0 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.1 +0.4 N.D. -0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.0 +0.3 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 +0.2 N.D. +0.3
growth on oleic acid YNB Oleic Acid +0.2 +0.2 +0.1 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.1 +0.0 N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 -0.0 +0.3 N.D. +0.1
growth on ypd YPD +0.3 +0.1 +0.3 N.D. +0.3
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